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定向克隆 的英文

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"定向克隆"怎么读用"定向克隆"造句
英文翻译手机版
  • directed cloning
  • directional cloning
例句与用法
  • The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
    应用逆转录-聚合酶链式反应( rt - pcr )技术克隆得到编码草鱼生长激素( cgh )的基因cdna ,并定向克隆到puc18载体上。
  • Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector , and e . coli bl21 was transformed by the recombinant plasmid for expression
    Pcr产物经纯化、酶切后,按正确的阅读框架定向克隆到表达性载体pgex - 6p - 1中谷胱甘肽转移酶( gst )基因的下游。
  • We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
    利用vp7基因和质粒pbi121上相同的单克隆位点,将vp7基因定向克隆到植物表达载体pbi121上,构建了pbi121vp7表达载体。
  • A plant expression vector was constructed by following method : s gene of 1bv and 35s promoter was cut from recornbinant pbi121 , and then the fragment was inserted into the multiclonal sites hind / bamh i in the plasmid pcambia1305 . 1
    通过从重组质粒pbi121上切下s基因(连同35s启动子)片段,将该片段定向克隆到pcambia1305 . 1质粒的多克隆位点hind 、 bamh之间,构建了一种植物表达载体。
  • For prokaryotic expression , the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ) . the gene was overexpressed in e . coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction
    用pcr方法扩增番红花八氢番茄红素脱氢酶( pds )编码区序列,定向克隆到表达载体pet一艺1a ( + )上,获得重组质粒pet一21a ( + ) / cspds 。
  • In the research of transgenic fish , green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology . thus pagfp plasmid was constructed successfully . the recombination was determined by digestion of restriction enzyme and sequencing
    实验通过分子重组技术,采用定向克隆法将绿色荧光蛋白基因亚克隆到puc118a上鲤鱼-肌动蛋白基因启动子下游,构建成能在真核生物体内表达的表达载体pagfp ,经双酶酶切法序列鉴定后,回收带启动子和目的基因片段。
  • Another 561 bp fragment of the orf of p22 gene was amplified by pcr , and digested by pst1 and xba 1 . the amplified fragment was cloned into pbudcfal to construct the recombinant plasmid pbudce4 . 1 / p22 . then transfected it into the murine macrophages by using lipofectamine , the expression of p22 - mrna in the transfected macrophages was identified by rt - pcr
    同时扩增p22编码基因orf的全长561bp片段,经pst 、 xba双酶切后,定向克隆于质粒pbudce4 . 1 ,构建真核重组重组表达质粒pbudce4 . 1 / p22 ,通过脂质体介导转染入小鼠巨噬细胞raw264 . 7 ,以rt - pcr方法鉴定目的基因在巨噬细胞中的表达。
  • Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease . the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning . through sds - page , bioactivity and function analysis of expressed protein , the function of phaa , phab and phac was confirmed
    菌株的亚克隆基因组片段中,分离出phaa 、 phab和phac三个基因片段,定向克隆至原核表达载体pbv220上,构建了三个原核生物表达载体pbv - a 、 pbv - b和pbv - c ,通过对表达载体诱导表达,表达蛋白产物的sds - page分析、生物活性与功能分析,确定了基因phaa 、 phab和phac的生物学功能。
  • Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris . by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides , a recombinant expression plasmid ppic9k - fl was constructed , and integrated into the alcohol oxidase region of the host genome
    为了提高外源基因的表达量,我们根据毕赤氏酵母偏爱密码子人工合成了编码fl胞外区156个氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表达载体ppic9k质粒上,构建ppic9k - fl表达质粒。
  • The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing . but difference was found at 3 bases of the sequence from the reported in genbank . then , an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404 . transgenic tomato were screened by their ability of growing on media containing kanamycin
    本实验采用pcr方法从番茄花cdna文库中克隆到叶绿体shsp基因,经测序证实与genbank中已发表的序列在编码区相差2个碱基,其中一个碱基导致1个氨基酸的改变。将叶绿体shsp基因定向克隆于带有组成性表达启动子camv35s的植物表达载体prok中,冻融法转化农杆菌lba4404 ,利用叶圆盘法对番茄进行ti质粒介导的遗传转化。
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