tca造句

• It is well known that the gear contact ratio has a close relation with the gears transmission error , contact pattern , load distribution , influences the gears meshing smooth , noise , dynamic strength and stiffness . based on development of the meshing theory , using tca , ltca and experiments , spiral bevel gears with high contact ratio are researched systematically
  众所周知，齿轮的重合度与齿轮传动误差、接触印痕与载荷分配密切相关，进而影响到齿轮啮合的平稳性、噪声、动刚度与动强度等。
• Main works and their important results are showed as following . firstly , the cdna encoding bullfrog gh is amplified by use of the total rna , extracted from adult bullfrog ' s pituitary , as the template , pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product , 660bp , was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method . after the purified cdna and pmd18 - t vector were ligated at 16 over night , the ligation products were transformed into e . coli strain dh5a and then the transformants were screened by clone pcr method
  首先，以成体牛蛙的脑垂体总rna为模板，进行rt ? pcr扩增得到约660bp的特异性pcr产物带，切下琼脂糖凝胶中的特异产物带进行cdna胶回收，将cdna与pmd18 ? t载体进行t ? a克隆连接并转化到dh5 e . coli后，进行菌落pcr 、质粒酶切鉴定，筛选出阳性菌株，测序结果经blast分析，与已报道的牛蛙生长激素基因高度同源，证实此阳性克隆为牛蛙生长激素基因转化子，命名为pbfgh 。