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ligase 中文是什么意思

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用"ligase"造句"ligase"怎么读
中文翻译手机版
  • n.
    【生物学】联结酶。
例句与用法
  • We have identified in s . cerevisiae distinct ubiquitin - ligase complexes that define different erad pathways
    我们在酵母体内发现了一种参与不同erad途径的特定的泛素连接酶复合体。
  • Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate
    连接酶:可将两个底物结合在一起的酶,此过程需要atp或其他核苷三磷酸供能。
  • Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
    将cdna与载体连接,并导入dh5感受态细胞中,构建成cdna文库。
  • As bases are added by polymerase to the starting point of a new complementary strand , known as a primer , or recognized by ligase as a match , the template ' s sequence is revealed
    当聚合酶将一个核苷酸加在新互补链的起始引子之后,或接合酶认定某段核苷酸链与原始模版配对,就可利用这些反应来得出原始模版的序列。
  • 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
    中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒,用kpni和bamhi酶切,分别回收cpti片断和酶切后的载体片段,用t _ 4连接酶连接构建成中间载体pgem - cp 。
  • Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli
    Hbsag质粒与ppiczaa载体分别经xhol和xbaln切,再在t4dna连接酶作用下进行连接,获得工程菌表达型ppiczaas ; hbsag质粒,转化大肠杆菌t0p10细胞,经xhol和xbal与sacll和xbal酶切电泳,证实s ; 。
  • Cut off beta fragment from plasmid prok . ii with hindlll and ecor i as insert , and cut pa into linear plasmid as vector fragment . link the insert and vector fragment together with t4 ligase , and the new vector with gene beta and gus was constructed
    用hind和ecor双酶切prok质粒,获得beta基因片段作为插入片段,用hind和ecor双酶切a质粒作为载体片段,将插入片段与载体片段相连,即构建成含有beta和gus的双基因载体。
  • At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
    利用vectornti6 . 0软件,对所克隆的序列用相邻接点法( neighborjoining州j ) method )进行多序列比对,分析其同源性,并构建基因进化树。
  • The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
    将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理, t _ 4dna连接酶连接,用连接产物转化大肠杆菌jm109感受态细胞,得到重组转移载体质粒pfastbac - gedna 。
  • This study demonstrated that the arabidopsis f - box protein coil associated with atcul1 , atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes . also , we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull . ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2
    用表达融合蛋白myc - ask2的拟南芥为材料,以- myc抗体进行免疫共沉淀分析发现, myc - ask2蛋白可以与coi1蛋白一起免疫共沉淀,但是不能与ask1蛋白免疫共沉淀,表明coi1蛋白与ask2蛋白,但是不能同时与ask1结合形成scf ~ ( coi1 )复合体。
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用"ligase"造句  
其他语种
百科解释
In biochemistry, ligase (from the Latin verb ligāre ? "to bind" or "to glue together") is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small chemical group dependent to one of the larger molecules or the enzyme catalyzing the linking together of two compounds....e.
详细百科解释
ligase的中文翻译,ligase是什么意思,怎么用汉语翻译ligase,ligase的中文意思,ligase的中文ligase in Chineseligase的中文ligase怎么读,发音,例句,用法和解释由查查在线词典提供,版权所有违者必究。