chloroplast genome中文什么意思
叶绿体基因组
- chloroplast: n. 【植物;植物学】叶绿体。
- genome: n. 基因组,染色体组。 adj. ...
- agranular chloroplast: 无基粒叶绿体
例句与用法
更多例句: 上一页 - To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c . reinhardtii transformants , pcr and pcr - southern blot analysis were performed using the primers of c . reinhardtii chloroplast homologous fragment
并根据衣藻叶绿体同源片段chll基因,设计一对引物对其叶绿体dna进行的pcr和southernblot分析。结果表明外源基因已经定点整合入衣藻叶绿体基因组的chll基因中,并且经过三轮抗生素筛选所得的衣藻叶绿体转化子中,转基因型叶绿体基因组拷贝占多数。 - To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity , the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast , respectively
经过两轮抗生素筛选后,采用fmdvvp1基因的一对引物和烟草叶绿体同源片段的一对引物分别对抗性植株的叶绿体dna进行的pcr和pcr - southernblot分析。 - In order to form a chloroplast transformation system of d . salina , we have conducted some studies including its sensitivity to antibiotics , the activity of promoter , cloning of the chloroplast genes and construction of transformation vectors , so far a pilot transformation system of the d . salina chloroplast has been completed . methods : the sensitivity of d . salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c . reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter . primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d . salina , and the sequences of 16s rrna , chll and chln of d . salina chloroplast were cloned and sequenced , respectively
方法:根据杜氏盐藻的近缘藻类的叶绿体基因组序列资料,在基因编码区的高度保守区域设计引物,克隆了杜氏盐藻叶绿体165识na基因、咖l基因和ch n基因,并分别以165识na基因和chln基因序列为同源片段,以cat和bar基因为筛选标记基因构建了三套杜氏盐藻叶绿体转化载体: 2郑州大学2003年博士学位论文杜氏盐藻( d ~ iiellasalina )叶绿体转化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ,用基因枪法转化杜氏盐藻,初步建立起杜氏盐藻叶绿体转化体系。