mab造句
例句与造句
- Through three rounds of screening , seventeen clones were selected and used in competitive test . the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera . based on the amin acid sequences deduced from the foreign sequence inserted in the phage , it was indicated that all clones shared the core sequence - p / ekphf , that was similar to aa50 ~ aa55 domain of n protein of prrsv
从第三轮亲和筛选的噬菌体中随机挑取17个克隆进行功能鉴定，结果表明8个克隆与mabge3具有较强的特异性结合力并可以被prrsv阳性血清阻断，测序发现7个克隆具有核心序列： p ekphf ，该序列与prrsvn蛋白aa50 aa55 ( p ekphf )具有较高的同源性。 - Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5 , rhpk - 5 ) protein was recognized by mab same as native hpk - 5 . the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5 . section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system , the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5
Coli )作为宿主，经sds - page分析，筛选表达量最高的菌株作为发酵用工程菌株；用western - blot方法鉴定hpk - 5因子的免疫学活性；用摇瓶发酵的方法，研究发酵培养基的体积(溶解氧) 、组成成份及诱导起始时间和诱导持续时间对目的蛋白表达量的影响，优化hpk - 5基因工程菌的表达条件。 - By using western - blot , the fusion protein could not be react with antiserum to prrsv , whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein
Western - blot分析表明表达产物与prrsv阳性血清没有反应性，而elisa分析表明表达产物可与prrsv阳性血清和单克隆抗体发生反应。由此说明单克隆抗体ge3所识别的抗原表位可能是存在于n蛋白上以kphf为核心的构象型表位。 - Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。 - It's difficult to find mab in a sentence. 用mab造句挺难的
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