pcr-rflp造句
例句与造句
- And pcr - rflp analysis was a sensitive , rapid and simple assay for the detection of nucleotide sequence change in ser 83 . and our data sugget that pcr - rflp may be a useful device for detecting the mutation in gyra gene associated the resistance to fqns
本研究通过试验条件的筛选，建立了pcr - rflp检测猪源致病性沙门氏菌gyra基因点突变的方法，可用于沙门氏菌氟喹诺酮类耐药性的分子水平检测和流行病学监测。 - Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali
本研究以从我国四川、河南、安徽和湖南等地分离的32株葛藤根瘤菌为研究对象，以20株已知种的根瘤菌为参比菌株，采用数值分类、 rep - pcr指纹分析、 16srdnapcr - rflp指纹分析等现代根瘤菌分类技术，初步研究了葛藤根瘤菌的生物多样性和分类地位，结果表明：葛藤根瘤菌在生长速率上表现出多样性，菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生长较快， yma培养基上28培养2 - 3天后，单个菌落直径大于1mm ，具有产酸能力，是快生型葛藤根瘤菌；其余待测葛藤根瘤菌生长较慢， yma培养基上28培养7天后，单个菌落直径小于1mm ，具有产碱能力，是慢生型葛藤根瘤菌。 - Taq i could distinguish p . abalonus and p . cystiodisus from the other pleurotus , but p . abalonus and p . cystiodisus could n ' t be differentiated for each other . msp i had the highest polymorphism and could divided 52 isolates of pleurotus into 8 groups , 5 , 5 and 4 groups for hae iii , hint ] , hha i in all isolates of pleurotus . cluster analysis based on pcr - rflp of 28s rdna 5 " half suggested that five groups were distinguishable for all isolates of pleurotus on 92 % similairity coefficient level , i . e . i p . djamor and p . salmoneoslramineus , p
28srdna5 ’端扩增产物的酶切分析表明，所有供试侧耳菌株无alu酶切多态性， bamlh可将金顶侧耳与其它供试侧耳分开， taq可将鲍鱼菇和囊盖侧耳与侧耳其它供试侧耳菌株区分开，而鲍鱼菇和囊盖侧耳则不能分开， msp酶切多态性最强，可将供试侧耳分为8种基因型， hae 、 hinf和hha次之，分别可将供试侧耳分为5种、 5种和4种基因型。 - In the present study , a portion ( the big intron between exon 5 and 6 , ca . 2 kb ) of glycerol - 3 - phosphate acyltransferase ( gpat ) gene of 15 wild tree peony accessions collected from 15 populations were analyzed using pcr - rflp technique and 9 accessions representing all of 8 species in sect . moutan were sequenced
本文对采自15个野生居群，代表牡丹组全部8个野生种的15份材料的gpat基因片段(外显子5和6之间的内含子)进行了pcr - rflp分析，并对代表牡丹组全部8个野生种的9份材料进行了测序。 - All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。 - It's difficult to find pcr-rflp in a sentence. 用pcr-rflp造句挺难的
- The miscellany appeared in strains of myrica , casuarina and alnus were also partly coincident with what normand et al . ( 1996 ) and li zhizhen ( 2002 ) obtained : the clusters devided by the isolates from the myrica and casuarinahave grest genetic diversities . besides the analysis of the strains , we also tried to extract the dna of frankia from the nodules directly and analyse them also with the method of pcr - rflp
这些结果与baker ( 1987 )通过交叉感染试验将来自不同宿主的50株frankia菌株分成4个类群的结果部分一致，但也暗示其可能具有局限性；此外，分析杨梅和木麻黄菌株所得到的结论支持了normand等( 1996 )与华中农业大学硕十研究生学位论文李志真( 2002 )所得的结论，即木麻黄和杨梅的菌株比较混杂，两者都不能成为独立的类群。 - Cluster analysis based on its pcr - rflp suggested that seven groups were distinguishable for pleurotus on 93 % similairity coefficient , i . e . p . oslrealus complex ( including p . ostreatus , p . florida , p . sapidus , p . corticalus , p . cornucopias , p . columbinus , p . spodoleucus , p . ferulae , p . nebrodensis and p . sp . ) , p . eryngii , p . pulmonarius - p
Itspcr一rflp聚类分析结果表明， 52个侧耳菌株在93 %的相似系数水平可分为七类，第一类包括糙皮侧耳、佛罗以达侧耳、美味侧耳、裂皮侧耳、黄白侧耳、哥伦比亚侧耳、灰自侧耳、阿魏蘑、白阿魏蘑;第二类为刺芹侧耳;第三类包括月 - Then the genetic distance and shannon information index among these 13 strains were obtained by using the software popgen2 on the basis of pcr - rflp patterns . the phylogenetic tree was constructed by using the upgma algorithm method ( mega2 software package ) . the shannon information index indicated that there was a higher genetic diversity among the frankia strains and some strains had a big genetic distance with others
根据酶切图谱，通过popgen2软件计算各供试菌株之间的遗传距离；用shannon信息指数计算种群遗传多样性；利用upgma法( mega2软件)对所获得的遗传距离矩阵进行聚类分析，得到13株供试frankia菌株间系统发育的聚类分析树状图谱。 - Some characteristics of cold - active protease and chitinase were analyzed then . microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences . the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area , as well as the interaction between microbial community and environment , were analyzed based on these studies
通过构建沉积物中微生物16srdna克隆文库，采用pcr - rflp分析、 dna - dna杂交、 16srdna序列测定以及系统发育分析的方法，研究了两太平洋“暖池”区和东太平洋“结核”区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系，得到了一些与“暖池”区环境特点紧密相关的新发现，新认识。
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