多拷贝的英文
mrna multicopy
multicopy
例句与用法
更多例句: 下一页- Gene duplication is defined as segment of dna duplicate one or more copies , it is believed to be a major mechanism for the generation of evolutionary novelty
摘要基因倍增指基因组中含有基因的dna片段复制出一个或更多拷贝的过程,是进化出新物种的主要原因。 - The whole gene was added every six hours instead of all in one time . in proper time , they were mixed and continued to ligate to multi - copy in the same direction
通过控制基因和接头加入的量及次序,可得到两侧有ecor和sal酶切位点的同向串连的多拷贝基因。 - Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting
培养多拷贝插入的酵母细胞,用甲醇诱导目的蛋白的分泌, sds - page和westernblotting鉴定表达蛋白。 - After linerization , the recombinant plasmid was transformed into pic hia pastoris by electroporation , which then were cultured in md plate free of histidine , from which the positive colones were propagated
重组质粒线性化后,用电击法将重组质粒转化入巴氏毕赤酵母,在缺组氨酸的md板上筛选阳性菌落,然后用不同浓度的g418 ? ypd板筛选多拷贝插入单菌落。 - 2 . southern blotting analysis indicated that sod2 gene had been integrated into all of the transgenic plants ' genomes . the copy number of sod2 in transgenic plants was mostly 1 to 5 by southern analysis
Southern结果表明,野生型植株(对照)没有明显的杂交信号,转基因株系有强阳性信号,转基因植株中插入外源基因的拷贝数不同,既有单拷贝的,又有两个拷贝及多拷贝的,拷贝数从1 - 5不等。