菌落原位杂交的英文

in situ colony hybridization

• 菌落:    bacterial colony
• 杂交:    hybridize; cross; ...
• mrna原位杂交:    mrna in situ hybri ...

例句与用法

1. In this paper , we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid
   以dig - oligo2为探针，菌落原位杂交筛选cosmid文库，得到56个阳性重组子。
2. Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned
   使用sau3ai对基因组dna进行不完全酶切，回收2 3kb片段，与puc18质粒连接转化大肠杆菌，利用地高辛标记探针，使用菌落原位杂交筛选转化子；筛选到包含有约1kb外源片段的转化子。