dmem造句
例句与造句
- culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清胶原酶消化法培养兔成骨细胞 - the culture results of the porcine ear skin fibroblasts was good by using dmem / f12 containing 20 % fetal bovine serum
在培养条件方面选取dmemf12培养液+20胎牛血清培养猪耳皮肤成纤维细胞，效果较好。 - the smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
应用胰酶消化法进行颌下腺细胞的分离、纯化及原代培养、传代培养。 - the viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6
方法无菌条件下，从6月龄新西兰白兔的膝关节囊内剪取滑膜组织，采用组织块培养法和酶消化法分离滑膜细胞。 - in the later experiments dmem-lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem-lg / atra ( 150mg / ml ) was used as the neuronal induction media
在以后的实验中，bfgf瞩0n才rnl）被用来作为前诱导剂，人ttiho50略inl）被用来作为诱导剂。 - It's difficult to find dmem in a sentence. 用dmem造句挺难的
- in the later experiments dmem-lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem-lg / atra ( 150mg / ml ) was used as the neuronal induction media
在以后的实验中，bfgf瞩0n才rnl）被用来作为前诱导剂，人ttiho50略inl）被用来作为诱导剂。 - to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs then transferred to neuronal induction media composed of dmem-lg / 1-10 mm bme
开始诱导时，将预培养基倒掉，细胞用pbs清洗后，转移人含有dmemlgl10mmbme的培养基中。 - mef were inactivated by treatment with 10ug / ml mitomycin c for 2.5-3 hours or y irradiation 50gy / 75gy dose and grew in dmem as feeder layer to help mouse es cells and human pgcs
采用10gml的丝裂霉素c或剂量为50gy和75gy的射线抑制mef有丝分裂，制作用于培养小鼠es细胞和人pgcs的饲养层。 - the cell-matrix complex was incubated with dmem and 10 % bovine serum under condition of 37, 5 % co2-the medium was changed daily . ( 3 ) cell-matrix complex paraffin section was made after 7 days incubation
将细胞悬液接种于戊二醛交联的胶原一壳聚糖多孔膜，加dmem10小牛血清培养基后放置37’c，5co。 - neuronal induction subconfluent cultures of mmscs were maintained in dmem-lg / 10 % fbs . 24h prior to neuronal induction, media were replaced with preinduction media consisting of dmem-lg / 20 % fbs / 1mm bme
2mmscs的神经诱导按以上方法制备细胞爬片，在神经诱导24小时前，培养基换成含有预诱导剂的培养基即合dmem?lg20fbslmmbme。 - neuronal induction subconfluent cultures of mmscs were maintained in dmem-lg / 10 % fbs . 24h prior to neuronal induction, media were replaced with preinduction media consisting of dmem-lg / 20 % fbs / 1mm bme
2mmscs的神经诱导按以上方法制备细胞爬片，在神经诱导24小时前，培养基换成含有预诱导剂的培养基即合dmem?lg20fbslmmbme。 - the clonies of es cells were flatter on the mitomycin c-inhibited sto fibroblasts, not easily differentiated from the feeder layer cells surround them, than that on the mef in dmem supplemented 15 % fbs . 5
以sto细胞作饲养层，添加15fbs，在倒置显微镜下观察，es-d3细胞不易与周围的sto饲养层细胞区别，形成的集落较平坦，生长状态不如mef饲养层好。 - methods isolation,, culture and purification of mouse mscs mmscs were isolated from the femurs of adult mice four weeks old and propagated in vitro . mmscs were originally cultured in dmem-lg with 10 % fbs, 100u / ml penicillion, 100mg / ml streptomycin
在细胞沉淀中加人含10胎牛血清的dmemlg培养基，计数细胞数，调整细胞密度，按3x10vinl接种于25cm‘的玻璃培养瓶中。 - result : 1 ) dmem : f12 would enhance the proliferation of hpfl, especially present with a concentration of 20ng / ml eof; pref feeder layer can defer the differentiation of hpfl in vitro, but collagen-vi and gelatin can not do
研究结果：1）含有egf的dmem：fiz对于hpfl的体外增殖明显优于其它培养液；在不同的培养基质中，pref饲养层优于明胶和胶原，可促进hpfl的生长，并能延缓hpfl在体外的分化。 - after transfected by sense and antisense pcdna3-doc-1r plasmid, we have also cultured mouse nih3t3 cells with dmem culture media containing g418 ( 400ug / ml ) for two weeks . using microscope, we have observed the plasmids'effection on a-bility of formation . 4
2?pcdna3一doc一ir重组表达载体的构建小鼠pme18s一f”一doc一ir质粒经转化扩增后，用xhal酶切消化pme18s一fu和pedna3质粒可分别得到975bp的doc一iredna片段和线性化的pcdna3载体片段。
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