pcr-sscp造句

"pcr-sscp"是什么意思   

例句与造句

  1. Pcr - sscp analysis on exon 1 of myogenin gene in sheep
    侧翼调控区的克隆与序列分析
  2. The application of pcr - sscp in forensic mtdna typing
    分型的法医学应用
  3. Polymorphism of brucella omp28 analyzed by pcr - sscp
    多态性分析
  4. Pcr - sscp analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep
    3基因外显子2的克隆与序列分析
  5. Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing
    方法利用酶切及sscp技术检测凝固酶基因序列改变情况,并测序进行确证。
  6. It's difficult to find pcr-sscp in a sentence. 用pcr-sscp造句挺难的
  7. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation
    将表达产物破包涵体后经glutathinonesepharose4b柱亲和层析分离, sds page显示纯化的蛋白质为一分子量60kd的单一蛋白带。
  8. With strong specificity and easy to manipulation , this simple , inexpensive , rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp , which shows better advantage than pcr - sscp . 2
    Mb杂交技术特异性强,简单快捷,能进行可视性检测,完全适用于点突变和snp的研究,技术特点明显优于pcr sscp 。
  9. The research results further prove that resistance to rifampin in mycobacterium tuberculosis resulted from the rpob gene mutation and pcr - sscp can quickly detect rifampin resistance in mycobacterium tuberculosis clinical isolates
    这些研究结果进一步证实,结核分枝杆菌耐利福平是由于rpob基因突变所致,采用pcr - sscp可快速检测结核分枝杆菌对利福平的耐药性。
  10. Compared with the results of dma direct sequencing , pcr - sscp has the same results as that except for failing to check out the rpob gene mutation of a rifampin - resistant isolate . it proves that pcr - sscp is a rapid and sensitive mutation testing method
    与dna测序结果相比较, pcr - sscp除一个耐药株的rpob基因突变没有检出外,其它检测结果均与dna测序结果相一致,这进一步说明pcr - sscp是一种快速、灵敏的突变检测技术。
  11. To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid , the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis , and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination
    在外显子5中,检出一杂合子突变,经dna序列测定证实为pro ( 207 ) leu突变杂合子,该患者为高甘油三酯血症病人。
  12. Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k . a 109bp fragment was amplified from the human genome dna . 2 . in 166 han samples , 19 ( 11 . 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
    用红细胞裂解液和pcr缓冲液非离于去污剂蛋白酶k两种自配试剂成功地提取了所收标本的人白细胞基因组dna ,并以此为模板成功扩增出预期的109hpmbl目的基因片段。
  13. This research began with detecting the rpob gene mutations of 37 mycobacterium tuberculosis clinical isolates by polymerase chain reaction - single strand conformation polymorphism ( pcr - sscp ) to study the application value of this method as a new molecule testing method for drug susceptibility
    本研究首先通过聚合酶链反应?单链构象多态性分析技术对37株结核分枝杆菌临床分离株的rpob基因进行突变检测,目的在于探讨pcr - sscp作为新的分子药敏试验方法的应用价值。
  14. By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ) , the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated . 3 . the molecular beacon ( mb ) hybridization technique with visual monitoring was established
    建立聚合酶链反应单链构象多态性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction , pcr sscp )方法,初步调查汉族人群mbl基因ggc54gac突变情况。
  15. Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
    具体方法为: ( 1 )照射后12h ,收集nih3t3细胞,用流式细胞仪检测各组细胞的细胞周期, pcr - sscp检测抑癌基因p16的变化; ( 2 ) nih3t3细胞照射后立即收集细胞和细胞上清,用试剂盒测量mda含量和sod 、 gsh - px的活性并观察其变化; ( 3 ) western免疫印迹和点杂交法检测照射2h后的各组hela细胞中基质金属蛋白酶- 2 ( mmp - 2 )的表达变化。

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