transformant造句
例句与造句
- The fermentation liquid of the transformant
转化子发酵液也具有脱毛效果。 - In contrast , most of the pollen grains in the non - transformant have plump shape and were stained into red color uniformly
转正向表达载体在烟草正常花粉粒的百分比较对照植株降低了42 。 - The transformant would flower in 8 to 10 weeks , sometimes under in vitro culture , after the leave dies was infected by agrobacterium tumefaciens
转化植株从浸染起8 - 10周可以开花，甚至在培养瓶中即可完成其生活史。 - And another change was that the spore of the transformant was difficult to release , p3a was not useful in genetically engineered strain construction in this way
此外， pcr扩增发现转化子比出发菌ybt - 1520多了cry1ab的特异带， sds - page电泳证实cry1ab的存在。 - Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification
通过pcr扩增表明，转基因植株中可以检测出有目的基因( ipt基因)的存在，目的基因已成功地插入菊花的基因组中。 - It's difficult to find transformant in a sentence. 用transformant造句挺难的
- Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。 - Transformant ybt - 833 - 1 kept all indigenous plasmids , ybt - 833 - 2 lost a plasmid carrying crylab and ybt - 833 - 3 lost all indigenous plasmids carrying icp genes . comparing their potency with natural strain ybt - 833 , the overall toxicity of all transformants was not noticeably higher than strain ybt - 833
质粒pbmblc电脉冲转入菌株ybt - 833 ，得到了几个icp基因背景不同的转化子，它们的综合毒力较出发菌ybt - 833都没能得到提高。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。 - Plant expression vector lba4404 ( pbin + - 35s - cbf3 + pal4404 ) was used in the genetic transformation of cucumber and micro - tomato . establishment of high efficiency regeneration system and determination of parameters involved in agrobacterium - mediated transformation , screening and characterization of transformant were systemically researched
将植物表达载体pbin ~ + - 35s - cbf3对黄瓜和小番茄进行遗传转化，建立了黄瓜高频再生和遗传转化体系，获得了一株转基因黄瓜植株，并通过了植物基因组pcr检测；获得了两个具有kan抗性的小番茄再生绿芽。 - T . identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis . both analysis the target bands were observed . so the integration of the cpti gene into mustard genome dna was confirmed . the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
转基因芥菜植株的鉴定取再生苗的叶片提取dna ，进行pcr扩增和pcrsouthernblot分析，转化再生植株大部分呈阳性，而非转化的再生植株均为阴性，证明cpt基因已存在于芥菜基因组中。 - 16 replacement plasmids for two different regions were obtained , but have n ' t been introduced into 10 - 22 successfully . 42 cosmids in this region were introduced into a heterogeneous host zx64 , one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton
将300kb区域的柯斯质粒进行异源表达，发现有一文库质粒5h4的转化子在硫链丝菌素存在时表现出对棉花枯萎病菌微弱的抑制活性；无硫链丝菌素存在时，则检测不到。 - The condition of somatic embryogenesis under different hormone conditions was discussed . the amount of embryo decreased but the amount of calli increased as the increase of kt concentration under the same 2 , 4 - d level after embryogenesis calli were transferred to medium with no hormone . the suitable concentration of kanamycin for selecting transgenic explants in the course of carrot tissue culture was screened in order to strictly select transformant
2 ， 4 ? d浓度相同kt浓度不同的激素组合随着kt浓度的升高，诱导的胚性愈伤转至不含激素的培养基上后胚状体发生量及发生胚状体的块数都随kt浓度增加而减少，但愈伤组织块的生长量却随kt的浓度升高而增加。
