The explants of for root , hypocotyl and cotyledon were cultured . among them the hypocotyls from 20d or 30d seedings was the best for callus forming and anthocyanin production 以20d或30d龄的无菌苗下胚轴作外植体，效果好，愈伤组织生长量大，花色素苷的含量也高。
An optimum ph for pm h + - atpase hydrolysis activity was around 7 . 0 , and the optimum temperature was 37 in vitro . thaw / freezing decreased the enzyme hydrolysis activity in our experiment 绿豆下胚轴质膜h ~ + - atpase水解活力受温度的影响， 37时，体外反应的水解活力最高。
In autograft of arabidopsis thaliana ( columbia24 ecotype ) grafted in the position of hypocotyls , wound xylem and sieve elements were differentiated in the tissues both of scion and stock 4 days after grafting C24拟南芥下胚轴自体嫁接后4d ，在接穗和砧木中就已有管状分子和筛分子的分化。
In all treatments of aseptic seedlings under 4 for different days , the treatment under 4 for 4 days produced the most amount ( 80 % ) of resistant embryogenic calli induced from hypocotyls 其中幼苗经4ac处理4天，从其下胚轴所获得的抗性愈伤最多，其抗性愈伤组织的诱导频率为80 % ，高于其它各处理的抗性愈伤组织的诱导频率。
Inversely , when kt was present in the medium the light induced inhibition was also decreased . it is an independent process of light and kt on inhibition of hypocotyl elongation and they showed multiplication effect on the process 不同光质下可降低kt对下胚轴伸长的抑制效应；反之， kt的存在也降低了不同光质对下胚轴伸长的抑制作用。
It is easy to induce callus derived from hypocotyls and cotyledons , but callus derived from root tip was rather difficult to be done . the most suitable medium for callus induction was the ms medium containing ba 0 . 5 mg / l and l . omg / l naa 愈伤组织的诱导与再分化的研究显示： “种都一号”儿菜下胚轴与子叶愈伤组织诱导较容易，但根尖很难愈伤化。
First , we studied the role of exogenous ca2 + ion in the hypocotyl phototropism . the curvature of hypocotyls was strongly depended on extracelluar ca2 + concentration . in the medium with low ca2 + ( < 200 nm ) , hypocotyl curvature was significantly inhibited 胞外钙离子对下胚轴向光弯曲影响的实验结果表明，不同的胞外钙离子浓度引起下胚轴向光弯曲角度发生变化。
3 effect of light quality on chlorophyll accumulation 2 - d - old seedlings grown under wl irradiated by wl , rl , fr , bl and uv - b irradiated for 3 d before the detection of chlorophyll content , it was showed that light quality influenced chlorophyll accumulation 光与kt对下胚轴伸长的抑制是彼此独立的的过程，当二者共同作用下胚轴时，会发生相互作用，二者主要表现出协同效应。
On the one hand , quercetin promotes hypocqtyls phototropic growth , on the other hand , it promotes gravitropism of root tips . when the seedlings are placed tilted at 80 , quercetin induces root tips getting into medium , while on control case , it grows along the surface 一方面q促进了下胚轴的向光生长，另一方面，促进了根尖的向地性，在80培养时， q诱导根尖钻入培养基中。
The average frequency of shoot regeneration in the cms line was 30 . 0 % and 2 . 34 % respectively and that in maintainer line was 30 . 0 % and 3 . 54 % respectively . on the other hand , explants of different sections of cotyledon resulted in different shoot regeneration abilities 不育系子叶与下胚轴的平均芽再生率分别为30 . 00和2 . 34 ；而保持系的平均芽再生率分别为30 . 0和3 . 54 。
