Infectious bursal disease virus has been a great concern for the poultry industry for a long time , particularly for the past decade when its " re - emergence " in variant or highly virulent forms . in this study , two sets of primers ( pta and pts , ibda and ibds ) , flanking the hyper - variable region of vp2 gene , were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr . both of these assays can amplify all of 12 reference strains which including pathotypes cibdv , vvibdv and vibdv , but not the 5 negative reference pathogens of chicken 结果12个参考毒株均能扩增出约679bp的目的片段，而阴性对照的常见5种鸡病病原：鸡新城疫病毒( ndv ) 、鸡传染性支气管炎病毒( ibv ) 、鸡传染性贫血病毒( caiv ) 、大肠杆菌和多杀性巴氏杆菌均没有扩增到任何片段；应用建立的技术对疑似ibd的34份临床病料进行检测，并同时在基础rt - pcr扩增的片段内设计另一对引物进行nested - pcr 。
Meanwhile , some transgenic plantlets and microtubers were transplanted into fields for reproduction and they survived . immunity assay with balb / c mice showed that expression product of transgenic potatoes with si gene of ibv had immunogenicity , and elisa antibody liter reached respectively 1 : 20 to 1 : 40 and 1 : 80 to 1 : 160 with doses of 0 . 5g and ig Balb / c小鼠免疫试验表明， 1克转基因马铃薯剂量免疫小鼠后， 21天的抗体elisa效价达1 ： 80 ? 1 ： 160 ，说明传染性支气管炎病毒s1基因的转基因马铃薯表达产物具有良好的免疫原性，这为ib植物疫苗的制备奠定基础。
The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared . by computer software , complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses , and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently 通过计算机分析软件，对我国ibv地方流行毒株lx4的主要结构基因全序列、分子特征及遗传变异进行分析比较，并初步分析预测传染性支气管炎病毒主要结构蛋白上可能存在的t细胞和b细胞表位。
