Serial paraffin sections were cut with paraffin microtome ( leica ) , thickness of which were 5 y m and 2 n m . mounting the sections on slides with protein and glycerol and then put them in 60 baker overnight Im ， ph7 2 ）磷酸缓冲液100一400 1 ， 30分钟灌注完毕，取出整脑，在上述固定剂koc ）内后固定12小时，切取观察部位脑块，将切块收入0
Other non - peroxidated aldehydes , such as formaldehyde , acetaldehyde , glyoxal , acrolein , pyruvaldehyde , salicylaldehyde , all did not change rheological property of these biological materials . the effects of mda on rheological parameters , crosslinking reaction and their relation with age pigment - like fluorescence were studied 戊二醛作为一种蛋白固定剂能特异地改变流体特性，而其它非脂质过氧化醛类，如甲醛、乙醛、乙二醛、丙烯醛、丙酮醛、水杨醛等并没有明显改变上述溶液的流变性。
Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces 2 ）磷酸缓冲液100400m ， 30分钟灌注完毕，取出整脑，在上述固定剂oc ）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still ， zlllll ，蛋自甘油载片捞片， 60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水， h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片， olympus万能显微镜照相。
