dna methylation

• I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids , to create a gene targeting insertion vector designated gfp tnos . after agro - infiltration into transgenic nicotiana benthamiana 16c , progeny were analyzed genetically for phenotypic changes , sirna accumulation , and dna methylation
  采用农杆菌浸润法( agro - infiltration )感染转基因本生烟16c ，并对同源基因瞬时表达所引起的植物表型变化、小分子rna的产生、 dna甲基化程度、以及相关性状在后代中的遗传情况进行了检查。