n-terminus

• On the other hand , our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells . the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells
  我们以往的研究表明，用绿荧光蛋白( gf内在信号肤下游n末端标记nrzb亚单位，与nri共转染时能形成与野生型相似的nmda受体通道，且可以通过抗gfp抗体标记活细胞膜表面表达的nmda受体复合物。