Nta hotel union is famous enterprises in shanghai to create the cuisine gateway piotr planning and design of the website interface fascinating cuisine styles , and unique visual experience is the focus of our pursuits Nta饭店联盟是沪上知名企业打造的美食门户，亚雷策划并设计了该网站的界面，引人入胜的美食格调和与众不同的视觉体验是我们追求的重点
Add 1ml of 50 % ni - nta slurry to 4ml lysate and mix gently and load the mixture into an empty column . then wash and elute the column with different buffer in order to purify the target fusion protein with the interaction of its 6 x his - tag and ni - nta matrices Rt一pcr扩增片断克隆于puc18质粒进行序列鉴定，结果显示所扩增cdna片断长13sbp ，序列分析与genbank中报道的编码hpd一3成熟肤的cdna序列完全一致。
Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein . after stepwise washing and elution at purification procedure , sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6 . 5 % 用ni一nta树脂亲和纯化表达产物，经过梯度漂洗和洗脱，获得纯度90 %以上的全长表达产物，另有6 . 5 %的非全长翻译产物(约70kda ) 。
Gfral was high expressed in e . coli after iptg induction . by ni2 + chelation affinity chromatography , the purified recombinant gfral protein were obtained . based on the evolutionary trace method , multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family 2 . gdnf的表达及其对pc12基因工程细胞的影响用本教研室已构建好的表达质粒pet一gdnf转化大肠杆菌bl21 ( de3 ) ，经lmmipto诱导gdnf表达，并在niz气nta柱上进行纯化，稀释复性后，纯度达90 %以上。
The distribution gis data management methods and its application in distribution running are systematically researched , which compose distribution gis network topology analysis ( nta ) and optimal rush - maintain path ( orp ) , and distribution planning , which composes distribution spatial load forecasting ( slf ) : ( 1 ) with systematic analysis on the relationship between spatial data model and spatial data structure , the distribution feature and the two common gis data models are analyzed , which are vector and raster data models . then the conceptual and logic data models of distribution gis are designed . the spatial data storage structure is given by using vector method , and their detailed data management methods are proposed （ 4 ）将gis的空间信息分析功能应用于配网空间负荷预测（ saptialloadforecasting ， slf ）的研究：综合利用灰色理论及遗传规划的优点，提出了一种根据馈线的历史峰值负荷进行外推的组合slf法：首先采用负荷转移耦合法来修正负荷历史，消除由于负荷转移引起的误差；然后根据实际馈线路径和配网gis图形分层，运用图层叠加分析得到馈线的实际供电范围；接着采用灰色关联度聚类方法对馈线负荷增长曲线进行聚类分析；最后采用遗传规划来对灰色聚类结果进行符号回归，分别得到每一类曲线的最佳拟合曲线形式。
The target protein purified by ni - nta metal chelate affinity presented one major band in the sds - page and reacted with rabbit serum raised against the ndv b95 strain in western blotting , suggested that the protein could be used as one of candidated antigenes . it provided the basis for further studying on recombinant vaccine against ndv 经westem - bloting杂交实验，纯化出目的蛋白与兔抗ndvb95株抗体反应，说明表达产物确为ndvb95株hn蛋白，且具有一定的免疫原性，为进一步研究重组疫苗创造了一定的条件。
