Chicken tyrp1 exons show 70 . 1 % nucleotide sequence homology with human exons . fish result shows that tyrp1 locates on the long arm of z and w near to the centromere 用荧光原位杂交进行tyrp1的染色体定位，首次将tyrp1定位在z和w染色体长臂近着丝粒的位置。
Through in situ hybridization and colony pcr , a positive recombinant plasmid was isolated from the genomic library and sequenced . the inserted dna fragment was about 4 . 3 kb 将pcr扩增片段用地高辛标记探针，利用原位杂交和菌落pcr从基因文库中获得含有阳性信号的重组质粒。
By computer image analysis system nyd - 1000 analysing the results of tunel and immunohistochemistry , accounting the size and positive cell of egg granulomas , apoptotic and positive index of granuloma cell was determined 原位杂交实验结果显示，实验纽b ； ix和尬l 。 rna表达匀高于对买组。
Herein , we used cloning , sequencing , northern blotting , in situ hybridization , in vitro translation and co - immunoprecipitation , aimed to investigate the expression of wnk genes and the function of wnk4 kinase 本实验应用克隆、测序、 northern印迹杂交、原位杂交、体外翻译和免疫共沉淀技术，旨在研究wnk基因的表达情况及wnk4激酶的功能。
During the embryogenesis the level of kyot mrna increases in 17 dpc and the distribution is as same as adult mice . immunohistochemical sabc and in situ hybridization analysis showed kyot was mainly located in leydig cells of testis of mice 采用免疫组织化学sabc和原位杂交方法进一步发现， kyot在小鼠辜丸中有特异性的表达，且特异性分布于睾丸间质细胞。
We obtained a fragment which showed 82 % similarity to cotton actin . it was used to prepare rna probe with dig labeling . distribution of actin mrna in root tip and callus was examined by in situ hybridization 在豌豆肌动蛋白基因编码区上切取同棉花肌动蛋白基因编码区同源性为82的一段序列为模板，进行地高辛标记合成rna探针，通过rna原位杂交技术对材料进行标记。
Clone human wnk4 full length cdna into pgem - t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7 - 27 ) and reverse primer ( 3808 - 3833 ) with the long template expanded pcr system kit 5 .原位杂交以小鼠肾脏总翩a为模板，扩增wnki基因外显子1 ， 24一28 ，以a片段和wnk4基因外显子13一16片段，纯化后连接在pgem一t载体上。
The aim of this study is to examine stat3 expression and activation in mouse uterus during early pregnancy , pseudopregnancy , delayed implantation , artificial decidualization and steroid hormonal treatments by situ hybridization and immunohistochemistry 本实验以小鼠为材料，利用原位杂交和免疫组化等方法，并采用假孕、延迟着床、人工蜕膜化及激素处理等模型，研究了stat3基因在早期妊娠子宫中的表达与调节。
In this dissertation , the plasmids containing 5s promoter were transfected into hela cells , the transcription sites of rna polymerase iii and its transcripts were detected by fluorescence in situ hybridization ( fish ) to dna , rna and dna - rna , respectively 本实验以人的hela细胞为材料，运用电击转染、荧光原位杂交并结合激光共聚焦显微镜，从dna 、 rna和dna - rna三个水平对rna聚合酶的转录位点及其转录子的分布进行研究。
In this dissertation , the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna , respectively 本实验以中国仓鼠卵巢细胞( cho )为实验材料，利用基因转染、荧光原位杂交并结合激光共聚焦显微镜观察的方法，在dna和rna水平上分别对rna聚合酶的转录位点和转录子的分布进行了检测。
