Studies on morphological observation and categorization showed that there were several differences between the strain m12 and the mutant strain m12 - 69 , but they both belong to monascus pilosus sato , according to hawksworth and pitt 第四，根据hawkswo曲和pitt关于红曲霉的分类方法，对m12及m12一69的培养物进行了形态观察及分类鉴定。
The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo , obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro 该毒株是将eiavl株（ eiavl ）通过驴体传代，使其毒力明显增强，然后在驴白细胞培养物上连续传代致弱获得的。
Reagents , microbiological media , cultures and other test materials , and samples awaiting testing must be stored appropriate to their stability profiles and requirements under conditions which prevent mix - up or cross - contamination and meet the ehs requirements 试剂、微生物培养基、培养物和其它试验材料，及待测样品的贮存条件必须符合其稳定性要求，必须防止混淆和交叉污染，必须符合ehs要求。
Three different batches of broomcorn millet cultures obtained under uniform conditions in millet processing , initial inocula level , and incubation were found not significantly differing in sporulation capacity . on non - nutritional agar plates , temperature significantly affected sporulaiton 本研究用白碳黑、硅藻土和变色硅胶对新鲜黍米培养物进行处理并在5和10下保存，均导致其快速丧失产抱能力。
Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly . 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so 3n ） ，将待测eaggec菌株的培养上清加入该报告菌株培养物中，用流式细胞术facs方法检测报告菌株荧光强度的变化情况，间接反映ybt的表达与否。
Detection of distribution of aii gene and aii protein in bacillus species and b . thuringiensis subspecies indicated that except for b . thuringiensis and b . cereus strains , b . subtilis and b . sphaericus strains had lower aii activity , but b . megalerium strain had no activity at all 用pcr方法也检测到，在上述四种细菌中存在aii基因。而巨大芽胞杆菌的培养物没有aii活性， southern杂交结果表明无aii基因的存在。
In this project , based on a great quantity of screening experiments , b . thuringiensis and b . cereus strains " culture was found containing a kind of protein which inhibits autoinducer activity . this protein was named aii and its encoding gene was named aii 本研究经大量筛选，发现苏云金芽胞杆菌和蜡状芽胞杆菌的培养物可抑制酰基高丝氨酸内酯( acyl - homoserine - lactone ，简称ahl )类ai分子的活性，其活性物质是一种蛋白质，称之为aii蛋白。
After the sequence and open reading frame were confirmed by dna sequencing , the gades gene was expressed in e . coli . sds - page analysis suggested that the bacteria bl21 ( de ) produce a kind of fusion protein of 92kd and the expressed gades fusion protein shows expected immunoreactivity by western blot Sds - page电泳和westernblot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50 ，相当于1 . 495g /每升培养物。
Steamed millets with water content of 45 % ( broomcorn ) and 42 % ( foxtail ) were inoculated with liquid culture of p . delphacis ( containing mycelial mass of ~ 25 mg / ml ) at a ratio of 20 % ( v / w ) and then incubated at 25 ? and l : d 12 : 12 将菌丝生物量约为25mg / ml的菌丝液按20的比例( v / w )接入经高温湿热灭菌并适度熟化、含水量分别为45和42的黍米及粟米中，在25和12l ： 12d条件下直接培养，所获3 17d黍米和粟米培养物的产孢潜能和有效产孢时间因培养天数不同而异。
Using erwinia carotovora strain scg1 as pathogen , the effect of b . thuringiensis strain 4d1 culture was detected on the rot soft disease . the results indicated that 4d1 culture inhibited the pathogenicity of scg1 in 24 hours when the cell density of scg1 was lower than odeoonm = 0 . 55 , but when the incubation time after infection was longer than 24 hours , the effect decreased 以胡萝卜软腐欧文氏菌菌株scg1为例，当菌株scg1的细胞浓度od _ ( 600nm ) < 0 . 55时，经苏云金芽胞杆菌菌株4d1培养物处理后感染马铃薯，在24小时内，可完全抑制软腐病斑，但随着培养时间的延长，其抑制作用逐渐减弱。
