A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis . a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ) , which was used as a probe for screening tomato fruit ( pink stage ) cdna library . one positive clone containing entire coding region were isolated , which was identified as a new member of acbf family by blast server , and named as leacbf 根据genbank (中的拟南芥、烟草acbf家族成员序列比较的结果，在该基因的保守区设计简并引物( degenerateprimer ) ，以转色期普通番茄果实的rna为模板，进行rt - pcr扩增，获得239bp的扩增片段，以此片段作为探针筛选转色期普通番茄果实cdna噬菌体文库，获得了包含全长编码区的阳性克隆。
The complete nucleotide sequences of two strain viruses were determined . ten pairs of primers were designed based on the nucleotide sequences of tbev sofjin - ho , oshima5 - 10 by dnastar software . the virus cdna was expanded by rt - pcr in ten parts . sequenced by clones method . 5 " terminal and 3 " terminal cdna was expanded by race method . the nucleotide sequences and deduced amino acid sequences of two strain viruses were compared with the sequences in genbank by dnastar software 根据genbank中已公布的tbevsofjin - ho株和oshima5 - 10株核苷酸序列利用dnastar软件设计10对引物，利用rt - pcr技术分段扩增病毒cdna ，克隆法测序。病毒基因组5末端和3末端序列采用race法扩增。测序结果用dnastar软件拼接，在genbank中检索6株tbev编码区核苷酸序列、 12株tbeve蛋白核苷酸序列，并在dnastar上进行核苷酸序列、由此推导的氨基酸序列比较及构建系统发生树。
The phylogenetic tree was constructed based on sequence comparison and analysis . the result showed that c strain derived from rabbit spleen tissue , c strain adapted to sk6 cell , shimen virulent strain all belonged to group 1 . 1 , and the prevalent virulent strains belonged to subgroup 2 . 1 or subgroup 2 . 2 . this indicated there were at least two subgroups , which were prevailing in china , and the prevalent virulent strains were diverse far from c . strain vaccine virus 通过序列比较和系统发生关系分析发现： c -株兔组织毒、 c ?株细胞毒和中国50 60年代流行的石门强毒株同属于组群1 ( group1 ) ；近期猪瘟流行毒株均属于组群2 ( group2 )的两个不同的亚组群( subgroup2 . 1和2 . 2 ) ，表明我国目前流行毒株至少有2个亚组群。
First , the process of default ratio model based on option - pricing theory was detailedly deduced and its applicability in china was analyzed and tested by taking the finances of sixteen st companies with default - record and the same amounts of 180 sample stocks listed in sse ( shanghai stock exchange ) in the same vocations as an example , and using the methods of cross - section comparison and time series comparison 文中重点介绍了基于期权定价理论违约率模型的推导过程；并以16家有违约记录的st公司和16家相同行业的上证180样本股作对比，通过截面比较和时间序列比较，分析检验了该模型在中国的适用性。
A pair of degenerate primers were designed in the conserved domain ( erf domain ) which based on the alignment of erebps gene family in tomato and other plant species . a 114 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ) , which was used as a probe for screening tomato fruit ( pink stage ) cdna library . two positive clones containing entire coding region were isolated , which were identified as new members of erebps family by blast server , and named as leerf1 and leerf2 本课题根据genbank中的拟南芥、烟草、番茄erebps家族成员的序列比较，在保守区( erf结构域)设计简并引物( degenerateprimer ) ，以破色期普通番茄果实rna为模板，进行rt - pcr扩增，获得114bp的同源片段，以此片段为探针筛选破色期普通番茄果实cdna噬菌体文库，获得两个包含全长编码区的阳性克隆。
The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr . sequence analysis of the gene was described in this papel the amino acid sequence , as derived from the nucleotide sequence of a cdna clone , had high homology with other subtilisin - like serine protease of nematogenous fimgi 与其他丝氨酸蛋白酶基因序列比较表明，与其他线虫卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性较高，而与捕食线虫真菌arthrobotrysoligospora同源性较低( 45 ) 。
2 . sequence comparison of alpha - amylase gene of bombyx mori and bombyx mandarina the alpha - amylase gene structures of bombyx mori and bombyx mandarina are very similar , both comprised of five exons and four introns , with comparable length . the mean at contents of exons and introns are not much different 家蚕和野桑蚕-淀粉酶基因序列比较分析家蚕和野桑蚕-淀粉酶基因具有相似的基因结构，都包含5个exon和4个intron ，各内含子、外显子长度在家蚕和野桑蚕间较类似，外显子和内含子at的平均值差异不大。
, as48 was identified to be the mouse sox4 homologue . ts47 may be a sox21 orthologue of trionyx sinensis , and , as47 may be a sox4 orthologue of alligator sinensis based on blast search . blast analyses showed that the percentage of identity at the amino acid level in the hmg - box region between ts - 41 , - 42 , as - 41 , - 42 and the non - reptilian sox - 1 , - 2 , - 3 was lower than that among the non - reptilian sox - l , - 2 , - 3 同源序列比较表明： as43 46和ts43 46与鼠sox12的hmg盒区有95的氨基酸同源性，表明是鼠sox12的同源基因( homologues ) ： as47 as48与鼠sox4的hmg盒区分别有100和97的氨基酸同源性，可以认为as47 48是鼠sox4的同源基因；与鼠、鸟sox21的hmg盒区相比， ts47分别有98和100的氨基酸同源性。
Comparison of the sequence of nucleotide and deduced amino acid of g gene of 119 and gxbm with that of 3ag and era vaccine strains , the homologies of nucleotide were ranged from 82 . 7 % to 84 . 0 % and 88 . 0 % to 91 . 6 % , homologies of amino acid has been showed 91 . 0 % , 88 . 8 % , 90 . 5 % and 88 . 0 % , respectively 将两株野毒株119 、 gxbm与人用疫苗3ag株和兽用疫苗era株的g基因进行了核苷酸序列比较，同源性介于82 . 7 - 84 . 0之间，推定氨基酸同源性在88 . 0 - 91 . 6之间，差异非常明显。
In addition , the evident crossing - infection happened in the strains came from myrica , casuarina also could be found in the sequencial analysis . all these results we obtained from the sequencing and rflp analysis were partly accorded with what baker brought forward in 1987 ( the four host specific group , hsg ) . however , they also indicated the limit of this hsg 5株供试菌株与其它已发表菌株的全序列比较结果可将所有菌株大致划分为4个簇，杨梅菌株fmr61 、木麻黄菌株fcg07和木麻黄菌株fce42具有较高相似性与桤木菌株聚为第簇；而杨梅菌株fmr16和2215与来自胡颓子科和沙棘的菌株聚在一起归为第簇。
