The who classification states that acute lymphocytic leukemia ( all ) should be classified by the pattern of reactivity of cell to a panel of lineage - associated antibodies and , where possible , genetic abnormalities . the expression of cd antigens on leukocytes is currently determined by flow cytimertry , which is expensive and labor - intensive , requiring 5 - 20ul quantities of fluores - cently labeled antibodies and allowing concurrent analysis for a limited number of cd antigens , usually three to four . from clinical point of view , an efficient method is required for analysis of a large number of samples in a single experiment 我们设计了一种新型的细胞芯片，其原理是充分利用玻片的硬度高，透明性好的特点，应用细胞膜表面不同的抗原物质，与包被在玻片上的不同抗体发生特异性结合，通过一次实验可以将被测细胞悬液中不同膜表型的细胞分离、固定在同一张玻片的不同特定区域，保持了细胞的完整性及活性，又可通过简单的操作一次将多细胞悬液进行免疫分群。
The content of this research include : screening the microorganism that can produce bioflocculant from the microorganism sample , select the best microorganism to optimize its culture condition that can increase the flocculating activity to the utmost ; in order to see about his flocculating activity , then using the bioflocculant flocculate the kaolin suspension and compare the flocculating results with the pac hpam ; on the basis of the bioflocculant has excellence flocculating activity , distill and purify the bioflocculant from the culture mediunu give qualitative analysis of its ingredient and reach its flocculating mechanism . the studies include the enrichment culture of the microorganisms that can produce bioflocculant and their screening suggest that they are broad exist in the soil and active sludge 本课题的研究内容包括：从微生物样品来源中筛选出微生物絮凝剂；从中挑选具有优良絮凝活性的微生物絮凝剂产生菌，对其产微生物絮凝剂的培养条件进行优化以提高其产絮凝剂的絮凝活性；用该絮凝剂絮凝高岭土悬液，并与聚合铝( pac )和水解聚丙烯酰胺( hpam )进行比较，以考察其对废水的絮凝效果；在具有优良絮凝活性的基础上，将该絮凝剂提取和纯化，对其进行分析研究，并对其絮凝机理进行探讨。
The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique . the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium , but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure . according the above result , the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end 这种亲水表面不仅有利于细胞悬液和培养介质的进入，并可以通过peo桥联的氨基酸( rgd )为细胞在三维多孔支架内的生长提供类细胞外基质环境；根据以上结果，本文对碱性氨基酸为peo链端基的两亲共聚物-氨基酸类细胞外基质修饰的聚乳酸三维支架进行了细胞相容性的测试。
Larval fish of cynoglossus semilaevis g unther were treated with colchicines to get the karyotype , 12 ~ 50 - day larval fish were selected to be put into the solution of colchicine of different concentration for 5 - 6 hours . the fin , and the other part of the fish were cut off to be treated with low concentration infiltration , solidify , eduction and other procedures . at last , the suspending cells can be gained and treated with air - drying method 选取孵化时间为12 50天的幼鱼，在不同浓度的秋水仙素溶液中处理5 6小时，分别剪取冠状幼鳍、鳃丝和身体周围的鳍条，通过低渗、固定、解离，得到细胞悬液，热片法滴片， giemsa染色。
Studies of the crystal structure of endostatin have shown a compact globular fold , with one face particularly rich in arginine residues acting as a heparin - binding epitope , this site was recently shown to be involved in the inhibition of induced angiogenesis . experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion . recombinant endostatin from bacteria is largely insoluble , but still efficient in arresting tu mor growth after injection into mice . intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors , including lewis lung carcinoma , to a dormant state . no sign of drug induced resistance has been reported and , in the original study , the treatment dormancy appeared to persist even when therapy was discontinued . sowe regard endostatin as a promising anti - tumor drug 许多研究表明重组内皮抑素特异性抑制内皮细胞增殖，而且这种抑制作用呈剂量依赖性。细菌表达产物内皮抑素大部分以不溶形式存在，将这种混悬液注射治疗老鼠仍可以抑制肿瘤生长。于小鼠皮下重复注射内皮抑素重组蛋白，几乎完全抑制鼠lewis肺癌等多种肿瘤生长，并无耐药性产生，即使中断治疗肿瘤也不再复发。
( 3 ) left lower limb was used to prepare artery ( red ) and vein ( blue ) color cast specimens using perchloroethylene . ( 4 ) deep freezed right lower limb sample , injecting red gelatin into femoral artery , forward and backward injecting blue latex with barium sulfate into femoral vein and great saphenous vein , cuting flap after dealing with formalin and get x - ray photos ( 3 )采用1例新鲜尸体左下肢标本制备过氯乙烯动(红) 、静(蓝)脉分色铸型标本。 ( 4 )右下肢冷藏标本，于股动脉灌注红色乳胶，再于股静脉及大隐静脉顺、逆行灌注含硫酸钡混悬液之蓝色乳胶，福尔马林防腐，然后切取皮瓣，摄取x线片。
