At present , the main methods used in the study on chinese traditional medicine in gastrointestinal dynamics animal experiment are gastric emptying and small intestine motional rate mensuration , parent electric bridge strain gauge mensuration , gut hormone determination ( gas , mot , ss et ) , immunohistochemical method , excised gastrointestinal smooth muscle dynamoscopy , intragastric and intestine pressure tracing 摘要中药在胃肠动力动物基础实验研究，目前主要采用胃排空小肠推进率测定法、胃肠道腔外电桥式应力传感器记录法、胃肠激素测定（胃泌素、胃动素、生长抑素等） 、免疫组织化学方法、离体体胃肠平滑肌肌条动力测定、胃肠内压描记法。
The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone . during the development of pancreas in alligator sinensis embryos , somatostatin ( ss ) immunoreactive ( ir ) cells , 5 - hydroxytryptamine ( 5 - ht ) - ir cells , glucagon ( glu ) - ir cells , epidermal growth factor ( egf ) - ir cells appeared on 18th day . no p53 protein - ir cell , gastrin - ir cell , testosterone - ir cell , chromogranin a - ir cell , vasoactive intestinal polypeptide - ir cell , epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos 本实验采用免疫组织化学技术，应用13种不同的抗血清，对孵育时间8 ? 55天扬子鳄胚胎消化道及消化腺内分泌细胞的种类进行鉴别、定位和比较，结果如下：扬子鳄胚胎胰腺中，生长抑素、 5 ?羟色胺、胰高血糖素、表皮生长因子、胰多肽免疫反应阳性细胞出现于第8天； p物质免疫阳性细胞出现于第18天； p53 、胃泌素、睾酮、嗜铬素a 、血管活性肠肽、上皮膜肮原、胰岛素免疫阳性细胞在各期扬子鳄胚胎胰腺中均未发现。
During the development of liver in alligator sinensis embryos , ss - ir cells appeared in a few of sinusoid endothelial cells on 8th day . 5 - ht - ir blood cells and tgf p 1 - ir blood cells were found in vein vessel from 30 - 55th day . egf - ir cells and sp - ir cells appeared in the outer membrane of liver from 40 - 55th day 扬子鳄胚胎肝脏中，生长抑素免疫阳性细胞出现于孵育第8天肝血窦内皮细胞间；孵育第30 ? 55天，静脉血管中发现5 ?羟色胺阳性血细胞和tgf 1阳性血细胞；表皮生长因子阳性细胞和p物质阳性细胞仅在第40 ? 55天出现于肝脏外被膜；甲胎蛋白免疫阳性细胞在第55天出现于肝脏外被膜及靠近外被膜的血管内皮。
