Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen , fibronctin , laminin ln , and the ecm metabolism influencing factors , including mmp2 , tissue inhibitor of metalloproteinase timp2 , transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system 动态检测各组大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次给药后测定大鼠血浆糖化血红蛋白hba1c透射电镜观察各组大鼠肾脏超微结构改变，应用免疫组化技术及图像采集分析系统测定各组大鼠肾脏组织中型胶原c纤维连接蛋白fn层粘连蛋白ln的表达，测定影响ecm代谢的基质金属蛋白酶2 mmp2基质金属蛋白酶抑制剂2 timp2及转化生长因子1 tgf 1的表达。
It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice , c57bl / 6 mice and goats obtained worm reduction of 50 . 46 % , 41 . 67 % and 42 . 78 % , respectively . np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s . japonicum . moreover , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter , area and volume of egg granuloma were all significantly less than those of control ; the contents of type i , iii of collagen and fibronectin were also significantly less than those of control ) 已对np30分子进行了较为广泛的研究，应用小鼠日本血吸虫肝肉芽肿模型证实np30对虫卵肉芽肿的形成具有致敏作用；对感染宿主(昆明种小鼠、 c57bl / 6小鼠和山羊)具有较好的免疫保护作用(减虫率分别为50 . 46 、 41 . 67和42 . 78 ) ；用np30主动免疫小鼠具有抗雌虫生殖产卵和抗卵胚发育的双重功效；另外，还对血吸虫病肉芽肿和肝纤维化有明显的负调节作用(虫卵肉芽肿的直径、面积和体积均明显小于对照组，肝组织、型胶原及纤维连接蛋白含量均低于对照组) 。
