Techniques of culture of prothoracic gland ( pg ) in vitro , 3h - ecdysteroid - radiounniunoassay , and methods of molecular biology and biophysics , for instance , nuclear magnetic resonance ( nmr ) , circular dichroism cd ) and so on were engaged . the biological activity on ecdysteroidogenesis in vitro and in vivo of a group of analog peptides of bom - ptsp synthesized by chemical modifications and the relationship between the structures and functions were studied 本研究利用~ 3h -蜕皮激素放射免疫分析方法、分子生物学、生物物理学的方法和手段、体外培养和体内导入等技术对bom - ptsp及其一组人工合成的与ptsp结构类似的多肽进行了结构和功能关系的研究，鉴定了它们的生理活性及其与结构的关系，以及它们在家蚕体内的活性及其与结构的关系，还对ptsp及其类似多肽进行了核磁共振和圆二色的研究。
