To a certain extent , the paper showed that there was not sure species - dependent relation between the in vitro culture and the establishment of the lines 因此从一定程度上表明， eg细胞的体外培养和细胞系建成并无绝对的品系依赖性。
This suggest that the in vitro culture doesn ' t affect their genotype , although dna sequence variations are present in the somaclone of " idaho " and the tetraploidy 可见，尽管组培苗与扦插苗有一定程度的变异，但并未影响到其基因型。
Advances in biotechnological research on chinese date were reviewed , which included in vitro culture , molecular markers and genetic engineering 本文综述了近年来我国枣生物技术的研究进展，主要包括离体培养、分子标记、基因工程3个方面的内容。
Tea cell line , derived from malignant keratinocyte , express keratin and is adopted widely in investigating the inhibitory mechanism due to its convenience for in vitro culture 经akautoah作用后， tea细胞的增殖显著抑制，且这种抑制呈抗体剂量依赖的方式6 ‘ 。
Response of barley anthers of 3 genotypes with higher tolerance and 1 genotype with susceptibility in vitro culture and callus derived to crude toxin of fusarium graminearum was investigated 摘要研究了在离体培养条件下，大麦耐、感赤霉病基因型花药对赤霉病菌粗毒素的反应。
With the development of in vitro culture and shoot regeneration from leaves , the method of agrobacterium - mediated transformation through leaf discs was widely adopted , and genes of interest have been transformed into strawberries 摘要随着植物离体再生技术的日益成熟和多种外源目的基因的分离与克隆，转基因技术以目的性强、周期短等优点成为草莓品种改良的重要途径。
However , genetic improvement by in vitro culture in mustard ( brassica juncea coss . ) , especially in chinese mustard , is restricted by the poor frequency of shoot regeneration , and no regeneration system is established yet 芥菜类作物(尤其是中国芥菜)再生率偏低，在遗传转化、突变体筛选等方面尚未形成一个高效稳定的再生体系，从而制约了离体条件下遗传改良的进程。
To select the best mouse blastocysts resulting from mm16 , mm16 + e2 , mm16 + p and mm16 + e2 + p mediums , and from ivf - ivc ( in vitro fertilization and in vitro culture ) in htf medium , and from in vivo was for embryo transfer 分别选取小鼠1 -细胞胚胎在mm16 、 mm16 + e2 、 mm16 + p 、 mm16 + e2 + p各组培养液中培养的囊胚、小鼠体外受精-体外培养的囊胚和体内正常发育的囊胚，进行子宫内移植。
According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo , the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere 摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手，阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求，寻求合理的体外培养条件和方法，以便提高体外胚胎早期的囊胚发育率。
Some interest has been generated in studying the rationale for assisted hatching . some reports suggest that embryos with lower zona pellucida thickness ( zpt ) and higher zona pellucida thickness variation ( zptv ) had a better chance of successful hatching and so the patient become pregnant ; another reports suggest that in vivo aging ; in vitro culture and exposure to ultralow temperature may induce zona hardening and then impair the embryo ability of hatching 有作者提出胚胎透明带厚度( zonapellucidathickness ， zpt )小的胚胎孵出时阻力减小，而透明带厚度变化率( zonapellucidathicknessvariation ， zptv )高的胚胎由于透明带上有一个明显薄的区域而使胚胎易于孵出；还有作者推测病人年龄、体外培养天数的增加及冷冻处理会增加透明带的硬度，使胚胎孵出困难。
