Interestingly , inhibition of p38 mapk activation ( but not erk activation ) by its inhibitors or by expression of a dominant negative mutant of p38 mapk could effectively block the chemotactic effect of ( - arrestin2 P38mapk上游激酶ask1的显性失活突变体也可以阻断- arrestin2对趋化的增强作用。这说明是p38mapk而不是erk信号通路与- arrestin2对趋化的增强作用相关。
Active mapks translocate into nucleus and thus regulate gene expression . now , three main parallel pathways of mapk family have been discovered : erk1 / 2 , jnk and p38 . they can induce different gene expression and thus cause different cell reaction 现已发现mapk家族主要有三条平行的信号转导通路： erk1 2 、 jnk和p38 ，三条通路可以活化不同的转录因子，启动不同的基因表达，从而引发不同的细胞反应。
In both cell lines ex - pressing sh2a gene , the cytoplasm pkc activity decreased 40 % , but no apparent alteration was found in mapk , tpk and membrane pkc activity . the proliferation index of transfected cells exhibited an decline in cytometry detection L sh的肝癌bel7402细胞及cos7细胞胞浆蛋白中的pkc活性均下降达40 ，胞膜pkc 、胞浆mapk ， ’ fpk活性未见明显改变，转染细胞经流式细胞仪检测增殖指数有所下降。
On the other hand , axudl mrna expression induced by tgf - 1 was not inhibited by inhibitor of mek1 / 2 or p38 mapk . these results suggested that tgf - 1 stimulated axudl expression required smads and was not dependent on activity of mapk signal passway 另一方面， tgf一pl诱导的axudl基因mrna的表达并不受mapk信号通路的meki / 2和p38两个激酶抑制剂的抑制，这一结果提示tgf一pl诱导的: xudl基因的表达需要smad3而非依赖于mapk信号通路的激活。
Many cell signal transduction pathways have been discovered in the regulation of apoptotic induction and reduction . mito - gen - activated protein kinase ( mapk ) is a group of particular ser / thr protein kinases which are activated when its conservative thr and tyr residues are both phosphorylated by upstream mapk kinases 丝裂原活化蛋白激酶( mapk )是一群特殊的丝苏氨酸蛋白激酶，它通过其上游mapkk使其保守的苏氨酸和酪氨酸残基双磷酸化而活化，活化的mapk可转移到核内，参与基因表达调控。
Although the intracellular mechanism of c . chinensis extract inducing differentiation of rat pheochromocytoma pc 12 cells remains to be clarified , the current results show that c . chinensis extract , enhancing the phosphorylation of mapk and inducing differentiation of pc 12 cells , might be involved in mapk - mediated signaling pathway 尽管细胞内的分子机制还有待进一步探索，但初步研究结果表明冤丝子提取物可能是通过激活mapk信号级联的某个上游步骤，活化mapk ，使其磷酸化程度提高，从而诱导pc12细胞分化。
There are two egfr signaling pathways , one is the classical ras / raf / mek / mapk pathway and the other is pi - 3 - k / pkb pathway . in this part , the phosphorylation and / or activity of some key molecules in these two signaling pathways in differently transfected h7721 cells were investigated . by means of specific antibodies combined with weste Egf刺激后不同转染细胞中的pkb活性、 p42 / 44mapk和mek磷酸化都明显增加，其中gntv一s / h7721细胞pkb比活性、 p42 / 44mapk和mek磷酸化程度较mock细胞增加明显，而gntv一as / h7721细胞中上述指标的增加程度则不及mock细胞。
Samples were taken from nonpregnant , pregnant and cultured endometrium cell , with using immunohistochemical staining , western - blot methods . to better understand this interaction , we further characterized the expression patterns of egfr messenger rnas in the mouse uterus on days 1 to 8 of pregnancy . in the present study , we found : two isofonns of non - phospho mapk was generally equally expressed in the nonpregnant and pregnant mice endometrium , and little difference among them 本研究以未孕及早孕小鼠( d1 ? d8 )的子宫内膜和培养的d4子宫内膜为样品，应用免疫组织化学、 western - blot等方法，检测了小鼠胚胎植入前后子宫内膜erk1 / erk2的表达部位及水平；用rt - pcr方法检测了egf及其受体egfr的表达情况；对培养的d4子宫内膜以le ~ y寡糖抗体阻断，观察了其对活性型mapk表达的影响。
There are still many questions remain unanswered , including the mechanisms that specify oligochitosan recognition , the participation of additional molecules in transmembrane signaling , and the repertoire of downstream pathways activated by oligochitosan . it was also observed that pdtc did not totally block no production , which gave us another evidence that there must be some other signal transduction pathways ( perhaps mapk , pkc , ca2 + dependent signaling ) involved in the activation , so further investigation is necessary to clarify molecular mechanism of oligochitosan inducing immunological en Westemblot结果显示100林岁ml剂量的壳寡糖作用于raw264 . 7细胞( 4x105个细胞/ ml ) 4h ，胞核内p65的含量明显增加， 6h时达到高峰， 10h时恢复到基础表达量，这就提示在壳寡糖对巨噬细胞的激活过程中nf一kb活化状态可以持续6个小时。
The related studies showed that the stress - activated protein kinase ( sapk ) and p38 mitogen - activated protein kinase ( p38 mapk ) signal transduction pathways are universally existing in the mammalian cells , which transduct the environmental physical stresses into cell and produce biological effects through the cascade phosphorylation between proteins 相关的研究表明， sapk途径及p38mapk途径是哺乳类动物细胞内普遍存在，并介导物理性应激的两条重要途径，它们通过蛋白质的级联磷酸化过程来进行信号的转导。
