Streptomyces whig gene is a key gene encoding a development ally important rna polymerase sigma factor ( awhig ) , which specifically initiates development program and determines the developmental fate of the cell Whig基因编码一个发育上重要的rna聚合酶因子，特异性地起始发育程序，决定菌丝细胞的发育命运。
In recent years , more and more scientists presumed that rna polymerase transcription might not occur in the nucleoplasm but in the nucleoli . nevertheless , the possibility has not been proved directly 近几年，有许多学者相继提出了rna聚合酶有可能在核仁区域内发生转录的观点，但是这一观点至今还没有得到直接的实验证据的支持。
Sequence data showed that the dna fragment containing a complete orf was 975bp , 68 . 3 % gc content . its amino acid product was a putative rna polymerase sigma factor , suggesting the orf was the true whig hologue 经测序和序列分析，证实该片段长975bp ， gc含量68 . 3 ，含有一个完整的orf ，编码278氨基酸残基，推测其蛋白产物是rna聚合酶因子。
Our results showed that , in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells , actin bound to brgl , nfl / ctf and rna polymerase ii during gene transcription . whereas , in unactivated mouse spleen t lymthocytes , no binding could be found 结果表明，在活化的小鼠脾t淋巴细胞和自主增殖的jurkat细胞中，肌动蛋白可以与mf复合物、转录因于nfi ctf和rna聚合酶11结合。
To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase 方法：根据端粒酶htert基因1573 ? 1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna 。
In this dissertation , the plasmids containing 5s promoter were transfected into hela cells , the transcription sites of rna polymerase iii and its transcripts were detected by fluorescence in situ hybridization ( fish ) to dna , rna and dna - rna , respectively 本实验以人的hela细胞为材料，运用电击转染、荧光原位杂交并结合激光共聚焦显微镜，从dna 、 rna和dna - rna三个水平对rna聚合酶的转录位点及其转录子的分布进行研究。
In this dissertation , the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna , respectively 本实验以中国仓鼠卵巢细胞( cho )为实验材料，利用基因转染、荧光原位杂交并结合激光共聚焦显微镜观察的方法，在dna和rna水平上分别对rna聚合酶的转录位点和转录子的分布进行了检测。
2 , immuno - co - precipitation showed that in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells , brg1 , nf1 / ctf and rna polymerase ii were closely bound together , and this was not found in un - activated cells 2 、免疫共沉淀实验证明，在活化的小鼠脾t淋巴细胞和自主增殖的jurkat细胞中， brg1 、 nf1 ctf和rna聚合酶是紧密结合在一起的，在没有活化的小鼠脾t淋巴细胞中这三种蛋白质没有免疫共沉淀现象。
The fragments were subcloned into a low copy transcription vector ( px8dt ) between the t7 rna polymerase promoter and autocatalytic hepatitis delta virus ribozyme . the result showed that genome of ndv f48e9 strain comprises 15192 nt , which was equal to zjl strain and 6 nucleosides longer than that of la sota , v4 , bl and clone30 实验结果表明： f48e9全基因组具有15192个碱基，比lasota 、 v4 、 b1和clone30的全基因组序列长6个碱基，和鹅源zj1株的长度相等。
It is well documented that they jointly take part in gene transcription that led by rna polymerase ii . however , whether baf complex and nf1 / ctf are also involved in the formation of rna polymerase ii - mediated transcription initiation complex is still uncertain , and a number of the currently proposed models still lack the support of experimental data 哺乳动物swi snf ( baf )复合物和转录因子( nf1 ctf )都是转录活动的重要调控因子，许多实验证实，它们共同参与了基因转录调控活动。 baf复合物与转录因子nf1 ctf是否一同参加了由rna聚合酶介导的基因转录起始复合物的形成，目前还没有比较一致的认识，已有的模型仍缺乏实验支持。
