Serum samples have been assayed and the results demonstrate feasibility of the proposed immunosensor for clinical analysis . ( 2 ) in chapter 2 , a renewable amperometric immunosensor has been proposed for the determination of schistosoma - japonium antibody ( sjab ) in rabbit serum . the experimental conditions of sjab determination were studied and optimized 结果与临床诊断用的elisa方法相比较，令人满意八2 ）在第二章中报导了制备可重复使用的日本血吸虫电化学免疫传感器，对r本血吸虫抗体（ sjab ）的实验条件进行了研究和优化。
1 . cloning of the gene encoding the specific ige antibody related antigen of schistosoma japonicum the serum samples from the inhabitants living in the epidemic regions of schistosomaisis japonica were collected and detected by abc - elisa method for their swap specific ige antibody . the sera with high titer of the specific ige levels were chosen and pooled 89 ，其亲水性、抗原性和可及性分布曲线基本一致，预测抗原表位分另ij在第25 35位、 75 82位、 1h 1们位、 117 1n位和149 159位氨基酸残基。
The second part , a renewable piezoelectric immunosensor is developed for the antibody of schistosoma - japonicum ( sjab ) . after incubating 32 kd molecular antigens of schistosoma japonicum ( sjag ) on the qcm by applying the immobilization above , the nonspecific sites on the immunosensor are sealed by using bsa and nrs together . the immunosensor can detect the sjab with the linear range of 0 . 54 ~ 32 . 50 ug / ml 以感染兔血清为检测对象，采用聚电解质吸附固定法，将日本血吸虫分子抗原( siag32kd )固定于石英晶振表面，再以牛血清白蛋白( bsa )和正常兔血清( nrs )联合封闭晶振上非特异性活性位点，可在0 . 54 32 . 50ug ml范围内检测感染兔血清中日本血吸虫抗体。
Np30 is regard as a candidate molecules of anti - pathology vaccine against schistosomiasis , however , anti - pathology immunity mechanism of np30 is still unclear . therefor , in this article , we studied the effect of np30 on apoptosis of egg granuloma cells in mice infected with schistosoma japonicum , and explore the molecular regulating mechanism of np30 Np30作为抗病疫苗的候选分子，但是np30抗病免疫作用的分子机制目前还不清楚，本丈从细胞凋亡角度研究np30对虫卵肉芽肿细胞凋亡的影响及其分子调控机制。
The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis 以往的研究业已证明np30对虫卯肉芽肿的形成具有致敏作用，并有十分显著抗虫卵肉芽肿肝纤维化作用，用np30主动免疫小鼠、山羊可减轻日本血吸虫对宿主造成的免疫病理损害。
It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice , c57bl / 6 mice and goats obtained worm reduction of 50 . 46 % , 41 . 67 % and 42 . 78 % , respectively . np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s . japonicum . moreover , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter , area and volume of egg granuloma were all significantly less than those of control ; the contents of type i , iii of collagen and fibronectin were also significantly less than those of control ) 已对np30分子进行了较为广泛的研究，应用小鼠日本血吸虫肝肉芽肿模型证实np30对虫卵肉芽肿的形成具有致敏作用；对感染宿主(昆明种小鼠、 c57bl / 6小鼠和山羊)具有较好的免疫保护作用(减虫率分别为50 . 46 、 41 . 67和42 . 78 ) ；用np30主动免疫小鼠具有抗雌虫生殖产卵和抗卵胚发育的双重功效；另外，还对血吸虫病肉芽肿和肝纤维化有明显的负调节作用(虫卵肉芽肿的直径、面积和体积均明显小于对照组，肝组织、型胶原及纤维连接蛋白含量均低于对照组) 。
Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
This thesis describes the preparation and " characterization of a renewable amperometric immunosensor based on sol - gel technology . renewable amperometric immunosensors have been developed for the complement 3 in human serum and schistosoma - japonium antibody , respectively . the detailed materials are showed as follows : this first part : renewable amperometric immunosensors have been developed with sol - gel as carrier 本论文报导了一种基于溶胶-凝胶技术的可更新的安培分析免疫传感器的制备和特性，分别制备了用于人血清中补体第3成份的测定和日本血吸虫抗体的测定。
Guan xiaohong , et al . ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum , whice is an internal image antigen of gut associated antigen ( gaa ) , its antibody isotype is igm . np30 has a partial cross - reaction with soluble egg antigen ( sba ) and membrane associated antigen ( maa ) 管晓虹等建立的日本血吸虫单克隆抗独特型抗体( anti - id ， ab _ 2 ) np30是肠相关抗原( gaa )的内影像anti - id ，与可溶性虫卵抗原( sea )有交叉反应，可替代虫源性抗原用于血吸虫病免疫诊断和抗病疫苗的研究。
