下胚轴

• ( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 )
  ( 1 )黄瓜子叶节植株再生建立东北农业大学理学硕士学位论文一通过对比三种外植体（于叶、子叶节、下胚轴）的分化率，得出最佳外植体类型为子叶节；通过探讨pgr （ naa 、 6 ba ）浓度和配比对不定芽分化的影响，得出最佳芽诱导培养基为：卜1叭6 。
• The highest vt and p valt in true leaf were accompanied two hybridizable polypeptides of aox protein , 35kd and 38kd respectively . the next was cotyledon vt and p valt with only one 38kd hybridizable polypeptide of aox protein . hypocotyl vt and p valt were the lowest and its immunobloting band was similar to that of cotyledon , but the expressive amount of 38kd protein was less than that of cotyledon
  绿豆幼苗不同器官的有关呼吸参数测定结果与aox表达的western分析基本一致：真叶的v _ t特别是v _ ( alt )最高，它也具有35kd和38kd的aox的杂交多肽；其次是子叶的v _ t和v _ ( alt ) ，且在子叶中，只见一条分子量为38kd的aox多肽；下胚轴的v _ t和v _ ( alt )都最低， western杂交显示也只有一条分子量为38kd的多肽，而且表达量也较少。
• It is concluded that the ca2 + influx from the apoplast through the ca2 + channel in the pm plays pivotal role in the hypocotyl curvature toward low - fluence bl . and we can give a further hypothesis that phototropin may act as the upstream regulator of calcium channel in the pm , from which the ca2 + influx and form the ca2 + singling as the second messenger in the cytoplasma
  综合以上的研究结果，本论文探讨了c扩+参与下胚轴向光弯曲反应中的可能作用机制，提出了质膜钙离子通道在photl介导的蓝光诱导下胚轴向光反应中可能的作用模式。
• Using the young seedlings of wild type ( wt ) and four mutants ( phya , phyb , phyaphyb and hy4 ) of arabidopsis thaliana as plant materials , the effects of light quality and kinetin ( kt ) on some photomorphogenesis responses such as cotyledon enlargement , hypocotyl growth inhibition , accumulation of chlorophyll and anthocyanin were investigated in the present study . photoreceptor functions and interaction of light and kt in the responses was examined and discussed
  本文以拟南芥( arabidopsisthaliana )野生型( wt )和四种光突变体phya 、 phyb 、 phyaphyb和砂hy4为材料研究不同光质与细胞分裂素( kt )对幼苗子叶扩大、下胚轴伸长、叶绿素和花色素苷积累的影响，分析光受体参与的反应以及探讨光与kt在调节各种反应中的相互关系。
• The results showed that the vessel element did n ' t differentiate after the seeds had imbibied for 6 hours ; 6 hours after seeds germination , two loci ? ne below the middle portion of the cotyledon and the other in the lower portion of cotyledon - node zone ( cnz ) initiated the differentiation of vessel element simultaneously , then elongated towards the cotyledonary apex and hypocotyl - root region respectively ; eventually the two promoter loci began to interconnect through vessel elements
  结果表明：种子吸涨6h ，未有导管分子的分化；种子萌发后6小时期间，自子叶中部偏下方和子叶节区下部的两个位点同时启动导管分子的分化，并分别向子叶叶尖和下胚轴-根的方向延伸；尔后，在发生两个启动位点之间发生导管分子的连接。
• The hypocotyl segments of cabbage were used as the explants and cultured on a ms medium supplemented with plant hormone , which was established a cabbage regenerated system . the differentiation rate of adventitious buds was above 80 % . in this study , ubic gene was transformed to cabbage through agrobacterium mediated system
  同时，采用甘蓝无菌苗的下胚轴为外植体，以ms为基本培养基，分别用直接分化再生系统和愈伤再生系统诱导不定芽的分化，建立了高效稳定的再生系统，不定芽分化频率达80以上。
• ( 3 ) the optimal explant age for transformation was 6 or 7 day after aseptic germination of seeds . ( 4 ) development of non - transformated cell was inhibited completely by 100mg / l kan . ( 5 ) 500mg / l cb could avoid pollution of agrobacterium tumefaciens and had no distinct effects on the formation of callus and shoot regeneration . ( 6 ) the optimal co - cultural condition was 3 day on 28 in dark
  以胡萝卜品种“改良黑田”为受体材料，以胡萝卜幼苗的下胚轴为外植体，建立了农杆菌介导法的胡萝卜转化体系，并对其中的种子消毒、激素水平、外植体的苗龄、选择压、共培养时间等关键步骤的条件进行了优化。
• Genes encoding the plant glutamate receptors were isolated from arabidopsis thaliana , a weed that has been adopted as a genetic model for plant researches . their study demonstrated that glutamate receptors in plants are also involved in a complex signalling pathway . when plants were grown in the presence of a compound that blocks human glutamate receptors , the plants response to light was partially hampered as illustrated by longer hypocotyl and reduced level of chlorophyll
  他们在拟南芥菜( arabidposisthaliana ,一种植物遗传学研究常用的野草)中分离出?氨酸感受器的基因编码，并发现这些感受器直接影响植物内的讯息传递当植物种植在含有?氨酸感受器抑制剂的培养基内，植物的感光反应即受影响，其下胚轴会增长而叶绿素的合成则减少，由此证明植物内光的讯息传递受?氨酸感受器调控。