The length of the 2nd intron sequences obtained by pcr amplification ranges from 153 to 168 bp in the 11 sisorid species ( 17 samples ) investigated , and is 154 bp in liobagrus kingi and liobagrus marginatoides 通过pcr技术，扩增了长度为154bp的金氏(鱼央)和拟缘(鱼央)以及长度为153 168bp的11种?科鱼类( 17个样品)的rps7基因内含子2序列。
However , someone has confirmed that a striking improvement both in the number of the transgenic mice that expressed the gene and in the average level of expression when the natural introns were included 但国外文献已经证实在转基因哺乳动物的研究中真核基因的内含子具有提高基因表达的能力，它能同时提高转基因鼠的成功率和基因表达的平均水平。
So , as well as spelling out which amino acids are needed to produce a specific protein , the part of the exon immediately next to the intron contains information that is essential for the gene editing process 因此，我们除了要弄清楚哪些氨基酸是产生某一特定蛋白所必需的外，还要弄清楚紧靠内含子的外含子里哪一部分含有基因编辑过程所必需的信息。
Therefore , we hope to construct a effective eukaryotic gene expressing vector harboring a genomic dna , including introns , and develop a gene expressing system could correctly splice the mrna 为此，我们希望构建和探索一种能有效高表达含有内含子真核基因组的载体和能对内含子进行剪切加工的昆虫表达系统，以提高外源真核基因表达的有效性和可靠性。
( 2 ) we searched for snps in 7 exons and intron 6 of tyrp1 by pcr and sequencing using genomic dna from 4 breeds . they are silky , white leghorn , blue shell layer and shouguang . there are 2 snps in intron 6 ( 2 )在丝羽乌骨鸡、白莱航鸡、绿壳蛋鸡和寿光鸡的tyrp1的7个外显子和内含子6中寻找snp ，比较pcr产物测序结果，只在内含子6中发现两个snp 。
Maize sbe2b gene has very high homology with that of rice , barly and wheat , and high alignment with maize ae gene with sequence no . l08065 . 1 . endosperm specific expression vector pebe2 was constructed 构建了无选择标记的sbe2b基因的表达载体，该质粒大小为6 . 06kb ，含有胚乳特异启动子，在该启动子的内部含有一个内含子，可在植物组织特异部位进行表达。
We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0 我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ，然后利用脂质体转染家蚕bmn细胞，瞬时表达hgh 。
Genescanning result in resource family with the microsatellite in intronl indicates that tyrp1 affects the color of skin and claw on a significant level ( 0 . 01 < p < 0 . 05 ) . and tyrp1 affects leg color on exteme significant level ( p < 0 . 01 ) 利用内含子1中的微卫星对家系进行基因组扫描，卡方检验结果表明tyrp1与皮肤色和爪色显著相关( 0 . 01 p 0 . 05 ) ，和胫色极显著相关( p 0 . 01 ) 。
The results showed that mod dna and cdna were 1378 bp and 853 bp in size respectively . for the first time , three introns were found in mod genomic dna by comparing dna to cdna , whose size were 315 bp , 123 bp and 87 bp respectively 测序西南农业人学硕士学位论文结果表明，人介gddxa大小为1378bp ，相对于edna853bp来说多了525bp ，在dna中首次发现了3个内含子，大小分别为315bp ， 123bp和87bp 。
The total efficiency of coinjection was 2 . 08 % . this study obtained the results and advances as follows : 1 . twelve transgenic mice were successfully produced by coinjection with three hum an dna fragments - hsa dna , hfenl and an uncoding cit987sk - 384d8 非编码大片段dnacit987sk 384d8对况a基因和hfeni基因整合有某种促进作用，这与内含子对基因整合有促进作用相吻合，也与brinster的研究结果一致。
