Based on modeling of the resultant time - dose - mortality data , the estimates of lc50 for the culture against the aphid species were 3 . 0 , 1 . 7 , and 1 . 2 cominia / mm2 on days 5 - 7 after exposure to conidial shower ; the estimates of lt50 dropped from 4 . 6 days at the concentration of 4 . 5 conidia / mm2 to 3 . 2 days at 26 . 3 conidia / mm2 . all aphid cadavers exhibited typical pandora syndrome . thus , the millet culture of p . delphacis was highly virulent to m . persicae , suggesting an applicability of the easy and convenient millet culture method to entomophthoralean fungi 用第1 、 5及9代黍米培养物对桃蚜2一3龄若蚜分别进行30min的单剂量抱子浴接种试验，均表现很强的侵染力，各代培养物的实际接种剂量分别为25 . 2个抱子/ mmz 、 18 . 7个抱子/ mmz和21 . 0个抱子/ mmz ，对桃蚜的lt50分别为3
The donkey - adapted strain of eiav ( dv116 ) , parental strain of eiav - dla , is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s . the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period . in this study , we constructed a full - length provirus dna clone of dv116 by pcr 本实验中将eiav驴强毒dv体外感染驴白细胞培养物，一定时间后收获病毒(本文中简称dv116 ) ，提取eiav前病毒dna ，以pcr法分别扩增并克隆了包含全长基因的三段前病毒dna片段，以双脱氧法测定了dv116病毒全基因序列共8236个核苷酸。
Sporulation on cultures of both millet species lasted for 6 days on non - nutritional substrate at 25c and l : d 12 : 12 . compared to 2 . 3 0 . 3 x104 conidia discharged from each cadaver of myzus persicae adults killed by p . delphacis with a < 60 - h duration of sporulation , 5 . 6 - 1 a and 3 . 7 - 4 . 7 times more conidia were discharged from each of the broomcorn millets cultured for 5 - 11 days and from each of the foxtail millets cultured for 3 - 11 days , respectively , with an over doubled sporulation duration 相对于新鲜蚜尸2 . 3 0 . 3 10 ~ 4个孢子/头的平均产孢量和60h的有效产孢时间， 5 11d黍米培养物的产孢潜能高出5 . 6 7 . 4倍， 3 11d的粟米培养物的产孢潜能则高出3 . 7 4 . 68倍，有效产孢时间均延长1倍以上。
The ammonia - oxidizers were increased from 2 . 13 106 / g mlss to 6 . 28 108 / g mlss , while the nitrite - oxidizers were increased from 2 . 13 103 / g mlss to 6 . 28 106 / g mlss . after a month , most of the heteotrophic bacteria were washed out from the system . they nitrifying bacteria were prevalent in the enrichment system and were around 99 % in total bacteria 选取富含硝化细菌的活性污泥作为富集培养的对象，采用纯无机培养基对硝化细菌进行定向富集培养，能在较短的时间内得到大量硝化细菌富集培养物，硝化细菌数约占总菌数的99以上。
In young chickens aev induces paralysis , ataxia and muscular dystrophy , while in older chickens , infection is usually subclinical , resulting in a decline in egg production and hatchability . infectivity was shown to remain unaffected by chloroform , low ph , pepsin , trypsin and deoxyribonuclease . magnesium cations were shown to stabilise preparations of the virus against heat inactivation . the buoyant density of virions are 1 . 31g / ml . the diameter of the virion was estimated to be 22 to 30nm . the aev can be adapted to grow in chicken embryo . the inability of aev to grow effeciently in most cell cultures 幼鸡感染该病毒后，引起麻痹、头颈震颤甚至共济失调，而成鸡常呈亚临床感染或导致产蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脱氧核糖核酸酶的影响，镁离子可增强病毒对热的稳定性，病毒的浮密度为1 . 31g ml ，直径为22 - 30nm ，该病毒主要在鸡胚中增殖，在大多数细胞培养物中不生长。
Twenty one adventitious buds from 30 root segment cultures were obtained after subculturing on medium lacking growth regulator for 90 days . the effects of hormones 6 - ba and naa on the adventitious bud differentiation were investigated and optimized . the results were showed that 6 - ba had significant effect on bud differentiation and the optimized medium for adventitious bud differentiation was ms + l . 0mg / l naa + 0 . 5mg / l 6 - ba . there were 67 adventitious buds occurred from average 30 hairy root segment cultures 90d后统计不定芽数量，平均每30个根段培养物上可获得21个不定芽；添加0 . 5mg 16 - ba能显著地促进毛状根不定芽的分化，配合使用6 - ba和naa对毛状根不定芽的分化最为有效( ms + 1 . 0mg lnaa + 0 . 5mg l6 - ba ) ， 90d后统计，平均每30个根段培养物上可获得67个不定芽。
Abstract : the total quantity of perylene quinonoids in hypocrella bambusae ( b . et br . ) sacc . , shiraia bambusicola p . henn and culture substance of ascomycetes hypocreacae ( fr ) tul . sp . were determined with spectrophotometric methods . the relative extraction efficiency were studied when ethanol , acetone and chloroform were used as solvents . among them acetone was the best one 文摘:用分光光度法测定了竹红菌、竹黄和菌寄生菌培养物中?醌类化合物的总量；同时研究了以无水乙醇、丙酮和氯仿为溶剂提取?醌类化合物的相对效率，结果发现以丙酮为溶剂最合适。
The present study was aimed to develop a solid - culture - based technology for easy and cheap propagation of p . delphacis f95129 inocula using small grains as substrate for fungal growth . sporulation capacity and timing pattern were relied upon to evaluate culture quality and identify potential factors to affect the quality . infectivity or virulence of inocula derived from the culture was assayed against the green peach aphid , myzus persicae ( sulzer ) , based on time - dose - mortality modeling 针对这一普遍性的技术难题，本研究以飞虱虫疠霉f95129菌株为对象，以黍米、粟米为基本材料，探索建立简便易行的侵染体繁殖技术体系，并对培养物进行了产孢潜能及其影响因子的评价以及针对桃蚜( myzuspersicae ( sulzer ) )的侵染性测定和时间?剂量?死亡率模型模拟。
In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not 合成该套式引物，选择大熊猫原代病料和病毒各代细胞培养物，经套式( nested ) rt一pcr扩增，可得到一与设计值5巧bp相符的dna片段，经bst一xl ( 590 ， 1110 )酶切鉴定，证明该扩增片断为特异性片段;回收大熊猫肝组织原代病料和细胞培养物第2 、 3 、 29代的ccvfz一ccvrz扩增片段，纯化，送生物公司测序。
