The first two reports of soybean transformation used two very different methods to transform soybean : hinchee et al ( 1988 ) used agrobacterium - mediated transformation of cotyledonary nodes while mccabe et al ( 1988 ) used particle bombardment of shoot meristems . soybean transformation reports following these initial papers have been limited and the transformation efficiency of soybean has remained low , the reason is that cells that are regeneration - competent must also be accessible and transformation - competent 目前应用于大豆遗传转化的方法主要有根癌农杆菌介导法，基因枪法，超声波辅助农杆菌法，真空抽滤法，花山东师范大学硕士学位论文粉管导人法等，但其中获得广泛应用的只有根癌农杆菌介导法和基因枪法，但是这两种方法在应用中也有一定的局限性。
4 . after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time , incubation time and co - culture time , we set up the system of high frequency transformation of tomato cotyledons . then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation , and the resistant plants to hyg were obtained . by pcr analysis on part of the putative transformants , we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants . 5 . transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg . trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection , growth and bio - morphology analysis , hyg - resistant experiment and pcr analysis 通过pcr检测证实部分番茄抗性植株中已导入hbmp - 3m基因；人骨形成蛋白一3成熟肤基因和全长基因分别转化番茄和烟草的研究5 .通过农杆菌介导法将hbmp一3全长基因导入烟草，并且获得了hyg抗性植株，通过gus基因瞬时表达检测、转化植株的生长情况及形态学分析、 hyg抗性鉴定及pc尺检测，证明目的基因己经整合到烟草基因组中。
Is a highly developed set of facilitation tools for enhancing participation and decision - making within an organization . these are utilized in executive planning processes and team development programs that result in new creativity , motivation and commitment 是一套发展完善的系列促导法，用来加强企业内部员工的参与精神及有效的制定决策过程这套技巧广泛地用在高级行政计划制定过程，和团队发展的项目中，结果总能在企业内部发掘出令人惊喜的全心创造力，激励和承诺。
Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle , immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens , the transformation rate of the above acceptor was investigated respectively . the results showed that immature embryo after precultured for 4 ~ 6d was the best . in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb , to agrobacterium tumefaciens eha 105 , od value of 0 . 8 was the best 采用农杆菌介导法将bt毒蛋白基因导入水稻同样以上述两种籼稻为主要研究材料，比较了分别以预培养的幼胚和幼穗、幼胚、成熟胚来源的愈伤组织作为转化受体的愈伤组织转化频率，结果表明预培养4 6天的幼胚最适宜作为农杆菌介导转化的受体；其次是来源于幼胚和成熟胚的生长状态良好的胚性愈伤组织。
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn 用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。
Hsp70 , a kind of molecular chaperone , has the main functions of taking part in protein folding , protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria . it has already been proved that there is a close correlation between hsp70 and the development of plants and animals . this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation . provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1 Hsp70是一种分子伴侣，主要功能是参与细胞有关蛋白新生肽的折叠、亚基组装、细胞内运输、蛋白质降解及dna损伤的修复，对线粒体结构和功能发挥重要作用，已有研究证明hsp70与动植物的发育有密切的关系，本研究将hsp70正、反义cdna构建成表达载体，并运用基因枪和农杆菌介导法将hsp70正、反义cdna导入烟草，试通过hsp70反义cdna抑制hsp70基因的表达从而创造雄性不育株，以证明hsp70反义cdna能创造雄性不育系。
With chip web server , embedded system can be monitored via ethernet , which can be used in remote measurement . for example , in our system , chip web server is used in conductivity measurement . remote file transport is very usful in embedded system design and debug , and its application is described in detail in section 4 在嵌入式系统中实现webserver ,用户就能在任意一台联网的计算机上,通过浏览器访问我们的系统,这就使远程监控、网络测量成为现实,我们已将其应用在基于双频测导法的电导率测量中。
In order to breed maize dwarf mosaic disease ? esistance maize species , the pap gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment , 10 transgenic maize plants were achieved and the pap gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods . the pap gene in one of the transgenic maize plants had been transcription and translated , the ability of the virus resistance is undergoing 为了解决玉米矮花叶病害的防治问题，采用花粉介导法及基因枪法将pap基因分别导入玉米的杂交种及自交系，获得了10株转基因玉米， pcr及southern - blot检测结果表明pap基因已经整合到了玉米基因组中，并且其中有一株pap基因已经得到了表达，病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。
Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility , with plasmid 3301 + 650 , 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding , 98 by agrobacterium ) . by cultivating them in blotting media containing basta 0 . 4 mg / 1 , we get 181 resistant leaves ( 98 by genegun bombarding , 88 by agrobacterium mediating ) 获得转基因雄性不育烟草为了证实hsp70反义cdna能创造雄性不育，我们将3301 + 650和3301 + 651质粒用基因枪和农杆菌介导法转化烟草无菌发芽的叶片，共207片(基因枪109片，农杆菌98片) 。在含basta0 . 4mg l的筛选培养基上进行筛选，得到抗性叶片181片(基因枪93片，农杆菌88片) 。
