Ten potential probiotics stains were tested in this experiment . including two lactobacillus acidophilus strains fbi and a878 , two bifidobacteria strains pba and inf , four enterococcus strains ml , pb2 , a30 , a31 . one bacillus abticism2 , one clostridium butyricum strain was isolated from commercial probiotics by using tpy 本试验共测定9株潜在益生菌，其中pb1和a878为嗜酸乳杆菌； inf和pba为双歧杆菌； m1 、 pb2 、 a30 、 a31为链球菌； m2为枯草芽孢杆菌。
The cold sunlight of this spring evening peered invidiously upon the crocks and kettles , upon the bunches of dried herbs shivering in the breeze , upon the brass handles of the dresser , upon the wicker - cradle they had all been rocked in , and upon the well - rubbed clock - case all of which gave out the reproachful gleam of indoor articles abandoned to the vicissitudes of a roofless exposure for which they were never made 春天傍晚清冷的太阳，好像含有恶意似地照射着那些坛坛罐罐，照射着一丛丛在微风中索索发抖的枯草，照射着碗柜的铜把手，照射到他们所有的孩子都睡过的那个摇篮上，照射在那座被擦得发亮的钟面上，太阳照射着所有这一切，这一切闪现着责备的亮光，好像在说，这些室内的物品，怎么会被扔到露天里来了。
- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms . the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product . in this paper , - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation , heat treatment , deae - sepharose fast flow column chromatography 本文对来源于枯草芽孢杆菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脱羧酶经硫酸铵分级沉淀、热处理、 deae - sepharosefastflow离子交换柱层析等分离纯化步骤，得到sds - paeg电泳纯，通过n末端氨基酸序列分析验证酶蛋白的纯度。
Extracts of 10 selected chinese herb medicines ( rhizoma coptidis , cortex phellodendri , radix scutellariae , fructus forsythiae , cortex magnoliae officinails , radix glycytthizae , folium artemisiae argyi , ligustici chuanxiong rhizoma , flos lonicerae , fructus schisandrae ) were tested in vitro against six different microorganisms [ bacteria ( staphylococcus aureus , escherichia coli and bacillus subtilis ) , yeast ( saccharomyces cerevisiae ) and find ( aspergillus flavu and trichoderma viride ) 摘要本文选用黄连、黄柏、黄芩、连翘、厚朴、甘草、艾叶、川芎、金银花及五味子十味中草药水提液，对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、啤酒酵母、黄曲霉及绿色木霉这六种菌进行了体外抗菌试验。
By transformation with the genes . plant disease biocontrol bacteria bacillus subtil is aplls and b . megaterium ap25 were isolated from wheat field soils collected from south australia and tai an . enzyme activity analysis on chitin agar and abp media showed that b . subtilis aplls secreted chitinase and b . megaterium ap25 secreted endoglucanase , respectively 测序后在genebank上进行序列比较，该基因片段同编号为2634966的枯草芽孢杆菌全序列的2599451到2812870 (功能未知)有85的同源性，但同已发表的13种几丁质酶的基因(包括枯草芽孢杆菌几丁质酶基因)的同源性很低，只有30 。
Four colonies of transformed e . coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained . the gene fragment in these isolates was identified by the methods of plasmid processing . dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b . subtilis from 2599451 to 2812870 was 85 % , and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b . subtilis ) in genebank 测序并序列比较结果表明该基因片段同已发表的枯草芽孢杆菌几丁质酶和内切葡聚糖酶编码幕因的克隆及重组芽抱杆菌的构建glyb一apre之间的同源性是最高的，为35 % ;同bacz ’了了ussp . bp23ce1b 、 b . p朋刀us内切葡聚糖酶和b . pol理vxap一1 ， 4一内切葡聚糖酶的编码基因的同源性只有27 % 。
Since the spring of 2001 , northern china has been hit by a severe drought , and as a result , brother tians family was recently left with over sixty acres of dry farmland without irrigation facilities . other irrigated farms were not doing any better , because the local mountain stream had long ago dried up . last spring , wild grass on the mountain was dry and yellow , a scene usually seen only in autumn 去年入春以来，大陆北方发生严重乾旱，田师兄家的六十几亩田都是旱田，没有任何灌溉设备，即使有灌溉设备的田地也好不到哪里去，因为山谷的溪水早就断流了，而山里的野草都已枯黄，放眼望去，一片如枯草连天的秋景。
The results showed that the extract by etoac presented a significant effect on staphylococcus aureau 、 bacillus cereus 、 bacillus megateriurn 、 proteus species , corynebacterium pekinense 、 trichoderma viride and aspergillus flavus all of which belonged to bacteria ; the extract by n - buoh presented significant inhibitory effect on bacillus mesentericus , bacillus subtilis 、 proteus species 、 bacterium prodigious , trichoderma viride and aspergillus flavus all of which belonged to bacterium ; the inhibitory effect become stronger and stronger with the extract concentration increaseing and the water phase of the extract did not present any antimicrobial effects 结果表明，乙酸乙酯萃取物对细菌中的金黄色葡萄球菌、蜡状芽孢杆菌、变形杆菌、巨大芽孢杆菌、北京棒状杆菌和霉菌中的绿色木霉、黄曲霉有明显的抑菌作用；正丁醇萃取物对细菌中的马铃薯芽孢杆菌、变形杆菌、枯草芽孢杆菌、灵杆菌和霉菌中的绿色木霉以及黄曲霉有明显的抑菌作用，且提取物的抑菌作用随浓度增大而增强，而水相则没有抑菌活性。
6 - phosphogluconate dehydrogenase ( 6 - pgadase , ec 1 . 1 . 1 . 44 ) was isolated by homogenate , ammunium sulfate fractionation , deae - sepharose chromatography , blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis , and some properties of the enzyme had been studied . a 113 . 8 - fold purification was obtained with a 8 . 2 % yield . the purified enzyme moved as a single electrophoretic band in page 将枯草芽孢杆菌超声波破壁后的粗提取物进行分段盐析、 deae - sepharose阴离子交换柱层析， blue - sepharosecl - 6b特异结合柱层析和sephadexg - 200凝胶过滤等纯化步骤，得到聚丙烯酰胺凝胶电泳为单一蛋白区带，比活为1 . 46u mg的酶制剂。
