Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin . the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5 . 4 ) . the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa . a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5 . 4 ) , which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ) . the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed . the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching 第一部分：等离子点ph ( 5 . 4 )条件下，用平衡透析法和紫外光谱，荧光光谱，共振散射光谱研究了ag ( )与人血清白蛋白( humanserumalbumin ，简称hsa )或牛血清白蛋白( bovineserumalbumin ，简称bsa )的结合。 scatchard图分析表明， ag ( )在hsa或bsa中有强弱两类结合部位，通过计算机拟合获得结合的逐级稳定常数值。紫外扫描发现ag ( )与hsa或bsa的结合存在滞后效应，表明ag ( )与hsa或bsa的结合可能诱导蛋白质构象发生缓慢变化( a - b ) ，测得并讨论了这一构象变化的速度常数和活化参数。
In this thesis the in situ brilliant cresyl blue ( bcb ) dimer with weak fluorescence in the solution of anionic surfactant ( as ) sodium lauryl sulfonate ( sls ) was used as fluorescence probe . at the same time , the new method for the determination of bovine serum albumin ( bsa ) was built . the linear range was 0 - 7mg / l and the detection limit ( 3 o / k ) was 3 . 66 10 - 3 mg / l , the r . s . d for the determination of 2 . 00mg / l bsa was 2 . 77 % 本论文以阳离子染料灿烂甲酚蓝在阴离子表面活性剂十二烷基磺酸钠存在下形成的现场弱荧光二聚体为荧光探针，建立了测定牛血清白蛋白的新方法，该法线性范围为0 7mg / l ，检测限为3 . 66 10 ~ ( - 3 ) mg / l ，对2 . 00mg / l几的牛血清白蛋白平行测定8次，相对标准偏差为2 . 77 ，说明本法准确可靠。
Anti - melatonin monoclonal antibodies of higher titer , affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections . we obtained 6 strains of hybridoma , all of them secreting specific antibodies to mt , we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique . an inhibition curve for mt was obtained in the range of 50pg to30ng , and 1 . 4ng of mt inhibited the value of the assay by half . we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues , the moabs recognized mt but 通过将mt用甲醛作连结剂连结到牛血清白蛋白上sa采用皮下多点注射兔疫小鼠得到了高效价，高亲和力，较好特异性的抗mt单克隆抗体，最后获得了5株单克隆细胞株，都能分泌针对mt的特异性抗体，建立了选择性基团免疫分析法，用制备的抗体测定了人血清中mt的含量，作了mt的抑制标准曲线，其抑制范围从50pg ? 30ng ，半抑制量为1
Part ii this paper has minutely studied the interaction between au ( iii ) and serum albumin . the binding of au ( iii ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 7 . 43 + 0 . 02 ) . the scatchard analysis indicates that there exists several strong binding sites of au ( iii ) in both hsa and bsa . a notable hysteretic effect has been observed in the interaction of au ( iii ) with hsa or bsa using uv - visible spectrometry at ph ( 7 . 43 + 0 . 02 ) , which shows that the binding between au ( iii ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for au ( iii ) to one of stronger affinity ( a - b transition ) . the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed . the binding equilibrium has project supported by the national science foundation of china ( no . 299621001 ) , and the foundation for talents of the ten hunred thousand in guangxi 第二部分：生理ph ( 7 . 43 0 . 02 )条件下，用平衡透析法和紫外光谱，荧光光谱研究了金( )与人血清白蛋白( humanserumalbumin ，简称hsa )或牛血清白蛋白( bovineserumalbumin ，简称bsa )的结合。 scatchard图分析表明，金( )在hsa或bsa中有强弱两类结合部位，通过计算机拟合获得结合的逐级稳定常数值。利用荧光猝灭法计算出金( ) - hsa和金( ) - bsa体系的stem - volmer常数和双分子猝灭速率常数。
