Based on capillary electrophoresis ( ce ) , mce chip is a multidisciplinary technology , which has roots in micro opto electro mechanical system ( moems ) , biochemistry and analytical chemistry . it became a new platform in the field of analytical chemistry , especially in the life science , drug discovery and medicine 它是毛细管电泳技术和微光机电系统、生物化学、分析化学等多学科的交叉，为分离分析领域提供了一种全新的技术平台，已经开始在生命科学、药物化学、医学等领域得到应用。
Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2 . the migrating distances of dna of brain , lung , spleen and kidney cells of mice increased significantly , compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0 . 2 % ) , and lead could strengthen dna damage degree by so2 in nuclear dna of brain , kidney , spleen cells . damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead 为了明确大气颗粒物中的重要组分? ?铅在二氧化硫所致dna损伤中的作用程度，利用单细胞凝胶电泳技术( singlecellgelelectrophoresis ， scge ，或称彗星实验， cometassay )研究了铅与二氧化硫的联合污染，结果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract铅单独及联合染毒条件下，小鼠脑、肺、肾、脾细胞dna迁移距离均比对照显著增加;铅加剧了50 :对脑、肾、脾细胞核dna的损伤程度; 50 :对肺细胞核dna的损伤程度要比铅的损伤大，小鼠肺细胞核dna迁移距离在50 :和醋酸铅联合作用组与醋酸铅单独作用组间有极显著性差异( p < 0 . 01 ) ，而与502单独作用组间没有显著性差异。
In order to understand the mechanism of mtx further and to investigate the genotoxic target organs , we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay . liver , spleen , bone marrow , thymus , kidney , testicle , stomach and peripheral lymphocytes of mice were isolated at lh , 3h , 6h , 12h , 24h after 5mg / kg mtx intraperitoneal injection 为了进一步了解甲氨蝶呤( mtx )的作用机制，探测其作用的遗传毒性靶器官，为应用mtx治疗过程中的临床监测和副作用防治提供理论依据，我们以小鼠为研究对象，用单细胞凝胶电泳技术检测了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后对肝、脾、骨髓、胸腺、肾、睾丸、胃和外周血淋巴细胞的dna损伤作用及损伤程度与mtx剂量间的关系。
