He drew back the bolt of his door , and almost directly an old lady entered , unannounced , carrying her shawl on her arm , and her bonnet in her hand . the white hair was thrown back from her yellow forehead , and her eyes , already sunken by the furrows of age , now almost disappeared beneath the eyelids swollen with grief 他打开门，进来了一位老太太，臂上挽着披肩，手里拿着帽子，不等通报就进来了白发压着她黄色的前额，她的眼睛周围刻满岁月留下的皱纹，眼睛几乎消失在那因悲哀过度而发肿的眼皮底下了。
The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis 以往的研究业已证明np30对虫卯肉芽肿的形成具有致敏作用，并有十分显著抗虫卵肉芽肿肝纤维化作用，用np30主动免疫小鼠、山羊可减轻日本血吸虫对宿主造成的免疫病理损害。
It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice , c57bl / 6 mice and goats obtained worm reduction of 50 . 46 % , 41 . 67 % and 42 . 78 % , respectively . np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s . japonicum . moreover , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter , area and volume of egg granuloma were all significantly less than those of control ; the contents of type i , iii of collagen and fibronectin were also significantly less than those of control ) 已对np30分子进行了较为广泛的研究，应用小鼠日本血吸虫肝肉芽肿模型证实np30对虫卵肉芽肿的形成具有致敏作用；对感染宿主(昆明种小鼠、 c57bl / 6小鼠和山羊)具有较好的免疫保护作用(减虫率分别为50 . 46 、 41 . 67和42 . 78 ) ；用np30主动免疫小鼠具有抗雌虫生殖产卵和抗卵胚发育的双重功效；另外，还对血吸虫病肉芽肿和肝纤维化有明显的负调节作用(虫卵肉芽肿的直径、面积和体积均明显小于对照组，肝组织、型胶原及纤维连接蛋白含量均低于对照组) 。
Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
