The main conclusions include : 1 ) among the non - biological factors , temperature is the most important factor that influence the development of the cochineal insect , the insect cannot finish life cycle under 15 associated conditions in spite of a few of egg can hatch , while at associated conditions of temperatures 20 , 25 , 30 , the insect can finish life cycle , but comparing from the amount of eggs , generation tendency indexes and the sizes of the female adults , 25 associated conditions are the best 得出的主要结论包括： 1 )在胭脂虫的生长发育过程中，温度是影响胭脂虫发育的最主要因子，在15的恒温状态下，尽管胭脂虫卵有少量孵化，但不能完成世代，而在20 、 25 、 30的恒温下胭脂虫能正常发育并完成世代。从培育的胭脂虫雌虫体大小、怀卵量及世代倾向等方面比较， 25的温度最有利于胭脂虫的培育。
Np30 is regard as a candidate molecules of anti - pathology vaccine against schistosomiasis , however , anti - pathology immunity mechanism of np30 is still unclear . therefor , in this article , we studied the effect of np30 on apoptosis of egg granuloma cells in mice infected with schistosoma japonicum , and explore the molecular regulating mechanism of np30 Np30作为抗病疫苗的候选分子，但是np30抗病免疫作用的分子机制目前还不清楚，本丈从细胞凋亡角度研究np30对虫卵肉芽肿细胞凋亡的影响及其分子调控机制。
The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis 以往的研究业已证明np30对虫卯肉芽肿的形成具有致敏作用，并有十分显著抗虫卵肉芽肿肝纤维化作用，用np30主动免疫小鼠、山羊可减轻日本血吸虫对宿主造成的免疫病理损害。
It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice , c57bl / 6 mice and goats obtained worm reduction of 50 . 46 % , 41 . 67 % and 42 . 78 % , respectively . np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s . japonicum . moreover , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter , area and volume of egg granuloma were all significantly less than those of control ; the contents of type i , iii of collagen and fibronectin were also significantly less than those of control ) 已对np30分子进行了较为广泛的研究，应用小鼠日本血吸虫肝肉芽肿模型证实np30对虫卵肉芽肿的形成具有致敏作用；对感染宿主(昆明种小鼠、 c57bl / 6小鼠和山羊)具有较好的免疫保护作用(减虫率分别为50 . 46 、 41 . 67和42 . 78 ) ；用np30主动免疫小鼠具有抗雌虫生殖产卵和抗卵胚发育的双重功效；另外，还对血吸虫病肉芽肿和肝纤维化有明显的负调节作用(虫卵肉芽肿的直径、面积和体积均明显小于对照组，肝组织、型胶原及纤维连接蛋白含量均低于对照组) 。
Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
Article 92 a train or other vehicle on which yellow fever cases are found on board upon arrival , or a train or other vehicle coming from the yellow fever prevalent area must undergo sanitization as stipulated in items 1 and 4 of article 89 of these implementation rules ; adult mosquitoes , their eggs and larvae on board must be annihilated ; the crew members and passengers without valid certificate of inoculation against yellow fever are required to undergo sanitization as stipulated in article 85 of these implementation rules 第九十二条对到达的时候载有黄热病病例的列车和其他车辆，或者来自黄热病疫区的列车和其他车辆，应当实施本细则第八十九条第(一) 、第(四)项规定的卫生处理；对列车、车辆彻底杀灭成蚊及其虫卵、幼虫；对无有效黄热病预防接种证书的员工、旅客，应当实施本细则第八十五条规定的卫生处理。
Guan et al ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 ofschistosomajaponicum , which is an internal image anti - idiotypic antibody of gut associated antigen ( gaa ) , its antibody isotype is igm . np30 has a partial cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) . np30 molecule has been researched on many aspects 管晓虹等( 1991 )建立了一株日本血吸虫单克隆抗独特型抗体np30 ，已证实为肠相关抗原( gaa )的内影像抗独特型抗体，其抗体同型为igm ，与可溶性虫卵抗原( sea )及膜相关抗原( maa )有部分交叉反应。
Guan xiaohong , et al . ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum , whice is an internal image antigen of gut associated antigen ( gaa ) , its antibody isotype is igm . np30 has a partial cross - reaction with soluble egg antigen ( sba ) and membrane associated antigen ( maa ) 管晓虹等建立的日本血吸虫单克隆抗独特型抗体( anti - id ， ab _ 2 ) np30是肠相关抗原( gaa )的内影像anti - id ，与可溶性虫卵抗原( sea )有交叉反应，可替代虫源性抗原用于血吸虫病免疫诊断和抗病疫苗的研究。
