Both the ph stability and thermostabilization of evolved a - aldc were higher than those of wild - type enzyme . this mutant is more suitable for the industrial production of beer . the replacement of arg results in partial disruption of the a - helix 并对天然酶和突变酶的酶学性质进行了分析，结果表明突变酶的ph稳定性和热稳定性均优于天然酶，更适合啤酒发酵。
Pretreatment with l - arginine ( l - arg ) before incubation with fac could not reverse the inhibition of relaxation response to ach completely . ( 2 ) in endothelium - intact aortic rings , l - arg could relax the pe preconstricted vessels （ 2 ）正常含有内皮的主动脉环在pe预收缩后，给予不同浓度的l afg可引起血管舒张；铁孵育后， l afg引起的舒张幅度显著减小（ p 0刀5 ） 。
In comparison with the results obtained with the control group , the concentrations of his , pro and ala in tobacco dried on the sheet metal decreased 30 % - 50 % , while those of asp , pro , lys , his , and arg in tobacco dried with high expansion dryer decreased significantly 而高温气流干燥与对照组则表现出明显的差异， 17种氨基酸的含量均有不同程度降低，尤其是如天门冬氨酸、脯氨酸、赖氨酸、组氨酸、精氨酸有明显的减少。
The results indicate that because of the improvement in two aspects mentioned above , the successful probability of the docking prediction is increased from unbound structures . for the case of enzyme trypsin - inhibitor appi , although the large conformational change occurs to the arg 15 side chain of appi upon complex formation , the native - like structure was still found and ranked first 其中，在胰蛋白酶appi复合物结构预测中，尽管抑制剂appi的15号残基arg侧链在复合物形成过程中发生了较大的构象变化，但其近天然结构仍然被找到了，并在打分中排在了第一位。
Part iv computer - aided molecule modelling of tnfa mimotopes and tnfa - binding peptides : to investigate the interaction between tnfa mimotopes and tnfa - binding peptides , the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt - 18 with tnfa firstly , then lcs - 7 was docked to llt - 18 by manual . the interaction between llt - 18 and tnfa or lcs - 7 showed electrostatic interaction and h - bond dominant 进行对接，对两者间结合位点进行分析；在此基础之上，以insight11软件构建lcs刁分于三维结构， autodock程序对接llt和lcs 7两个短肽分子，找寻两者结合的关键性残基，结果表明llt与tnfa及lcs 7分子间相互作用以静电相互作用为主， lcs刀和tnfa活性部位的arg在分于间相互作用中起重要作用。
A mixture of three amino acids ( arg , gly , glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip , the separation voltage is 200v / cm , the separation time is less than 120 seconds ; according to ccd fluorescence images , two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b , a kind of temperature - dependent fluorescence dye , was used as probe to develop a temperature - fluorescence intensity equation , then temperature - color map in microchannels was constructed , and temperature trait in microchannels on the pdms microfluidic chip was analysed . according to the results , we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm 利用pdms微流控芯片对fitc标记的精氨酸、甘氨酸、谷氨酸混合物进行了电泳分离，分离电压为200v cm ，分离时间不到120秒；通过拍到的荧光显微图像对电泳注样过程中复杂的样品分子积聚与解聚现象作定性的分析；以荧光染料rhodamineb为温度荧光探针，建立了pdms微流控芯片上的温度-荧光强度的关系公式，并利用matlab图像处理工具箱构建出微流体沟道内的温度色图，对pdms微流控芯片的微流道温度特性进行了分析，根据实验结果，我们认为对于pdms微流控芯片来说，在进行需要外加电场作用的试验时，外加电场不应超过400v cm 。
Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells . only after adhering to surface followed by spreading can cells develop and proliferate . most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity , which may correlates to the low degree of cell attachment . modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation . cell attachment to substrate is primarily mediated by integrins , a widely expressed family of heterodimeric surface receptors . most extrcellular matrix proteins such as fibronectin , osteopontin , collagen type i , bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin . the main aim of this research is to measure , assess adhesion , proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin , collagen type i or biotie gen , which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture . ( 2 ) adhesion , proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed . ( 3 ) also , adhesion , proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin , or collagen type i respectively . ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo . this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities 因此将这些蛋白包被、固定到材料表面，观察骨组织工程种子细胞mscs细胞的粘附、生长特性是本研究的中心环节，并从以下方面进行探讨： ( 1 )采用不同原代细胞分离方法，研究其对mscs细胞的生物学特性影响。 ( 2 )检测基因胜肽胶对mscs细胞粘附、增殖及分化的影响。 ( 3 )分别采用型胶原及纤维粘连蛋白( fibronectin ， fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycolide ) ， plga )膜及多孔块型plga材料，观察细胞在单层或三维培养状态下，型胶原及fn对mscs细胞粘附、增殖及向成骨细胞分化效应及能力。
After a maximal response to kc1 was obtained under an initial tension of 2 g , isometric contractions induced by iron or pe were measured , and calculated as percentage of the maximal tension induced by kc1 . the rings with or without endothelium were preconstricted with 10 " 6mol / l of pe and a cumulative concentration - response curve to ach , or sodium nitroprusside ( snp ) , or l - arginine ( l - arg ) was obtained 用10 ~ ( - 6 ) mol lpe预收缩后，加入不同浓度的ach 、硝普钠( snp )或l -精氨酸( l - arg ) ，浙江大学硕士学位论文以各种浓度所致的舒张幅度达到pe所致的收缩幅度的百分比作血管舒张反应的量效曲线。
We used gradient dilution renaluration ( renaturation buffer : 0 . 14mol / l tris - hcl ph8 . 0 . 10mmol / lgsh , 2mmol / lgssg . 5 % glycerol , 0 . 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively . to get pure nk4 , it was purified by gst affinity gromatochraphy . to detect the angiostatic activity of nk4 in vivo , it was tested using cam 6 )诱导温度对pg厂x一4t一l一nk4表达菌株bl21表达目的蛋自的影响分别进行了25 ’ c 、 300c 、 37温度状态卜，以iptg化学诱导，发现其均可表达，全菌表达量: 37最高，其次为30诱导时。
Because the large conformational changes occurring upon complex formation are frequently confined to the protein surface , especially for the side chains of flexible amino acids arg , lys , asp , glu and met , the locations of the mass centers and the values of radii of these residues are modified for modeling protein surface . this tolerates overlap in some degree between the amino acid side chains on the surfaces of the receptor and the ligand 由于蛋白质表面氨基酸残基arg 、 lys 、 asp 、 glu和met具有较大的柔性，因此，北京工业大学工学博士学位论文一我们对蛋白质分子表面的这五种氨基酸模型作了修正，调整了残基球心的位置和半径的数值，使分子表面的氨基酸残基在对接时具有一定程度的可交叠性。
