Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e . metck darmstadt methods superovulation and collection of eggs for superovulation , female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ) , and after 46 - 48 hours with human chorionic ginadotropin . ( hcg ) . one - cell fertilized eggs were collected on the next day from oviduct of females 取4一5周龄成熟雌性昆明系小白鼠，腹腔注射pmsg (孕马血清促性腺激素) 10iu ， 46一48小时后腹腔注射hcg (人绒毛膜促性腺激素) 1oiu ，将注射hcg后的雌鼠与8周以上的成熟雄鼠合笼交配，次日检察阴栓，将查到阴栓的雌鼠处死，取输卵管于mz培养液中，解剖镜下撕开壶腹，释放细胞团，然后用300林岁nil透明质酸酶消化去除颗粒细胞，口控吸管将卵细胞在m :中反复清洗，然后置于孵箱中，根据时间点收集g2期细胞。
As the most common used small experimental animals , rat and mouse have inconsistencys in reproduction , especially in gondonatorpin inducing oocyte maturation . our research was undertook to elucidate which kind of gonadotropins play role in oocyte meiotic maturation , the effects of fsh ans lh / hcg on mouse and rat oocyte maturation in vitro were examined under different culture models 在体外自发成熟模型中，大鼠的去卵丘裸卵( do )自发成熟发生要较卵丘包被的卵母细胞( ceo )早， do培养2h时gvbd可达到88 ，而ceo培养2h的gvbd为53 。
