After washing with reagent ( 50mmol / l ph8 . 0 tris - hcl , 100mmol / l nacl , 0 . 5mmol / l edta , 2mol / l carbamide , 0 . 2 % triton x - 100 , 0 . 2 % doc ) , ultrasound crushing ( 200 w , 150 times , 3 seconds per time , spacing 3 seconds ) and freezing - melting methods , we got hng fusion protein and m - insulin fusion protein with purity of above 80 % . 4 . radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0 . 5 unit per litre bacterial liquid 3 .表达产物初步纯化仁gly ”一flumanin融合蛋白和人胰岛素突变体融合蛋白包涵体的洗涤方案为: lj ]体沉淀溶于含zmol ， / l尿索洗涤液，超声( 200w 、巧o次、 3秒/次、间隔3秒) ， 4 、 i000orpm离心15min后沉淀用洗涤液充分重悬，一2 ( )冻存过夜，次日融化后4 、 10000rpm离心15min ，取上清，即得到初步纯化的融合蛋白，可去除土要的杂蛋白，融合蛋白溶解于上清液中，纯度达到80 %以_ 1 : 。
Dissecting the prothoracic glands , by using silkworms " prothoracic glands in vitro culture and h - ecdysteroid - radioimmunoassay , the biological activities of those analog peptides were examined , and their active features , chemical parameters , as well as the relationship between structures and functions of those peptides were analyzed and compared . with an exception of 899808 having the sequence of awqdlsaw that affected like bom - ptsp , 899805 ( awqdlns ) and 899809 ( awqdlnsgaw ) partially stimulated ecdysteroidogenesis in a dose dependent manner , whereas 899803 ( dlnsaw ) , 899804 ( awqdlnsa ) , 899806 ( awqdln ) as well as 899807 ( awqdlnsawa ) had both stimulatory and inhibitory effects on ecdysteroidogenesis , depending on the concentrations 研究结果表明，类似多肽89980吕（ awq uawaw ）的生物学功能与抑前胸腺肽的相同而且活性近似：类似多肽899805 （ awqdlns ） 899809 （ awqdlns … ）对家蚕前胸腺蜕皮激素的生物合成表现出随浓度增加而增加的促进作用，而低浓度下几乎不促进； 899803 （ dlnsawawl899804 （ awqdmsa ） 、 899806 （ awqdln ）和899807 （ awqdlnsawa ）类似多肽对家蚕前胸腺蜕皮激素的生物合成的促进和抑制作用与它们的浓度有着依赖关系。
2 . to establish a neutrajizing - inhabition time - resolved fluoroimmunoassay ( trfia ) of hepatitis b virus e antibody ( hbeab ) and hepatitis b virus core antibody ( hbcab ) based on the competion . the assay ranges of standard cures were 0 - 16ncu / mland 0 - 8ncu / ml . the within - run coefficient variations for standard samples were less than 10 % . compared this method with radioimmunoassay , the sensitivities were 92 . 8 % and 93 . 3 % 以中和抑制法建立了乙型肝炎病毒e抗体时间分辨荧光免疫分析法( hbeab - trfia )和乙型肝炎病毒核心抗体时间分辨荧光免疫分析法( hbcab - trfia ) ，标准曲线分析范围分别为0 - 16ncu ml和0 - 8ncu ml 。
Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2 , signal molecules , skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ) . radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation . ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method 应用免疫组织化学方法光镜下观察高渗刺激后，大鼠视上核胶质细胞(星形胶质细胞和小胶质细胞)受体( nmdar2 ) 、信号分子、骨架蛋白及缝隙连接蛋白的表达的时空变化；应用放免测定检测高渗刺激前后血浆中vp含量。
The author organized four volleyball match experiments , in which 12 ( 6 males and 6 females ) are wuhu juvenile volleyball players , and 12 ( 6 males and 6 females ) are students majoring in volleyball from college of re , and tracked these participants in soft - volleyball matches to measure the amount of alb and b2 - mg in their last urine before and after each match by means of radioimmunoassay 作者对芜湖市青少年排球运动员(男、女各6人)及体育学院排球专选班学生(男、女各6人)分别进行了四局正式比赛的运动实验，并跟踪了实验对象参加的软式排球赛，以放射免疫法检测了实验对象在各次运动前、后终尿中alb ， _ 2 - mg的含量。
With anti - t - jgy establishing a radioimmunoassay , the quality of anti - t4 igy was appraised : litres was 1 : 25 00 , permitting rang of litres in the ria was from 1 : 2500 to 1 : 5000 ; the degree of cross reactivities with ta was 1 . 28 % ; affinity constants was 2 . 13 x 109l / mol 本文应用蛋黄正丁醇去脂获得的抗t4 igy建立了放免检测方法，对抗t4igy的质量进行鉴定：抗体滴度为1 ： 2500 ，工作浓度在1 ： 2500至1 ： 5000范围内；与t3的交叉反应为1 28 ；亲和常数为2 13x109l m 。
In this study , we use immunohistochemistry , electron microscope , cell culture , image analysis , mtt method and radioimmunoassay to study the localization , varies of quantitation , and possible frictions of 5 - ht and subtype of 5 - htr in human placenta . the results were as follows : 1 本研究用免疫组织化学、免疫电镜、细胞培养、图象分析、 mtt法和放射免疫等技术对5 -羟色胺及其受体亚型在胎盘绒毛中的细胞定位及对滋养层细胞增殖和激素分泌的影响进行了初步的探讨，其结果如下： 1
This paper describes techniques for detecting early markers of dn , including routine methods , such as radioimmunoassay , chemical luminescence immunoassay , enzyme - linked immunosorbent assay etc . , and biosensors and microelectrochemical biosensors , such as piezoid sensor , fluorescence biosensor , surface plasmon resonance biosensor etc 本文介绍糖尿病肾病早期标志物的检测技术,包括放射免疫测定法、化学发光免疫测定法、酶联免疫吸附测定法等实验室常规检测方法,以及石英晶体免疫传感器、荧光生物传感器、表面等离子体共振生物传感器和微型电化学生物传感器等检测技术。
