Pairwise comparisons of uncorrected sequences distances and kimura 2 - parameter distances were calculated and molecular phylogenetic trees were reconstructed by using the neighbor - joining ( nj ) , maximum parsimony ( mp ) , maximum likelihood ( ml ) methods 分析了?科鱼类s7核糖体蛋白基因内含子2序列，计算了p - distance和kimura2 - parameter遗传距离，并用邻接法、最大简约法、最大似然法重建了?科鱼类分子系统树。
It was suggested that the gene t13m11 . 8 could be the ast candidate gene . this gene was 1432bp long with 6 exons and 5 introns . the putative protein of t13m11 . 8 gene was highly homologous to dihydroflavonol 4 - reductase ( dfr ) , which was an important enzyme in the anthocyanin biosynthesis pathway 该基因的dna序列长1432bp ，含有6个外显子和5个内含子，编码的蛋白与花青苷生物合成途径中的二氢黄酮醇4 -还原酶有较高的同源性。
Based on cdna sequences of ( 3 - carotene ketolase ( cr / ff ) and p - carotene hydroxylase ( c / - fz ) , a 1 . 6 kb crtw genomic sequence with six introns , and a 3kb crtz genomic sequence with five introns were clone , respectively . all the exon - intron junctions conform closely to gu - ag consensus splicing rule 本论文工作成功地克隆两个关键酶基因的基因组序列，发现crtw和crtz分别包括6个和5个内含子序列，所有这11个内含子的剪切位点都符合gu - ag规律。
The genomic dna sequences of rvde genes were also amplified through pcr . jrvde gene has four introns as 105bp , 327bp , 81bp and 69bp , respectively , in its encoding region . in contrast , the introns of irvde gene are the same as that ofjrvde except that the second is 425bp 通过pcr扩增获得了rvde基因的核基因组dna序列，在它们的编码区中含有4个内含子，其长度在jrvde中分别为105bp 、 327bp 、 81bp和69bp ，而irvde基因的第2个内含子长425bp ，与jrvde的第2个内含子差别较大。
