2 . although calculating magnetic - flux leak coefficient and magnetic - reluctance coefficient is too complex to be done , combining magnetic force pump alnico ' s characters , appropriate accounting models are selected to calculate these coefficients through magnet conductivity methodology , the equation has been induced 虽然漏磁系数和磁阻系数的计算非常复杂，利用磁导法，结合磁力泵磁钢排布特点，选择适合磁力联轴器特点的计算模型，给出了漏磁系数的计算公式。
In this study , we constructed two plant expression vector with rip gene from barley and linked to two promoters which have high promoting efficiency in monocotyledonous plants , respectively , and then transferred to rice mediated by agrobacterium tumefaciens . the main factors of affecting transformation were investigated and established its regeneration and transformation systems . pcr analysis showed primarily that rip gene had already integrated into rice 本研究构建了分别由单子叶植物中启动效率较高的pact和p2 35s启动子调控的大麦核糖体失活蛋白基因( rip )的植物表达载体，通过农杆菌介导法转化水稻，经pcr检测初步验证了rip基因已导入水稻。
4 . the expression vector was introduced into young embryo - derived calluses of weeker winter - type wheat variety " youngmail58 " by agrobacterium - mediated and biolistic transformation , and 15 gus - positive plant lines were regenerated . the transformation rates by agrobacterium - mediation and biolistic bombardment were 1 . 23 % and 1 . 71 % , respectively 将所构建表达载体分别用农杆菌介导法和基因枪法导入半冬性小麦品种“扬麦158 ”的幼胚愈伤，共获得15株gus阳性苗，其中农杆菌法的转化效率为1 . 23 ，基因枪法的转化效率为1 . 71 。
