Abstract ： this review introduced the research process of the establishement of genetic transformation system in maize . the method and integration and expression of exogenous genes were also discussed 摘要本文介绍了近年来玉米遗传转化系统的建立、基因导入手段、方法等方面的研究进展。
At the same time , we transfered chs to quamocliy pennata by agrobacterium mediated and established genetic transformation system of quamocliy pennata through the guide of gus reporter gene 并以农杆菌介导将chs导入茑萝中，初步建立了茑萝的遗传转化系统，为今后茑萝遗传改良的进一步研究奠定基础。
The best concentration of acetosyringone was about 30 m . silver nitrate below 4 mg / l caused little change in efficiency and higher concentration ( above 10 mg / l ) strongly inhibited hairy root induction M时，转化率不能显著提高。 4m幼以下的硝酸银便能有效地提高遗传转化率，但高剂量( 10m幼)时作用却相反。
Bar gene also is one of widely used selective marker gene . the cloning of bar gene is important to plant transgenic engineering , gene expression and studying physiology 而且bar基因也是迄今为止应用最为广泛的一个抗除草剂选择标记基因，克隆出bar基因对植物的遗传转化，基因表达和生理学研究都有重要的作用。
Owing to the cofinement of the host of agrobactrium tumefliens and the difficulties of the maize inbred lines for obtaining embryogenic calli , the genetic system of maize was n ' t ideal enough 由于农杆菌介导玉米遗传转化受到农杆菌宿主的限制和玉米自交系获得再生植株难的影响，使玉米自交系遗传转化体系仍不完善。
But the genetic transformation of xylophyta is far lagged behind that of most agronomic species , mainly because lack of a high efficient system of regeneration and genetic transformation 绝大部分果树属于木本植物。果树的遗传转化进展缓慢，甚至落后于林木，其原因仍然是缺乏适宜的遗传转化受体和高效的遗传转化方法。
