Study on nodulation and nitrogen fixation of azospirillum brasilense induced by 2 , 4 - d in the roots of maize 诱导固氮螺菌在玉米根部结瘤及固氮效果的研究
The effect of chitosan coating preservative with 2 , 4 - d , chlorine dioxide on grapes stored at room temperature was studied 摘要用含2 , 4 - d 、二氧化氯的壳聚糖涂膜剂研究葡萄的常温保鲜效果。
The generation of protein 55kd was regulated by 2 , 4 - d and the protein increased the frequence of somatic embryogenesis obviously 55kd蛋白组分受生长素2 ， 4d的负调控，并对体细胞胚的发生具有显著的促进作用。
Study on controlling weeds in soybean land by covering soil surface with sprays of 50 ec of prometryne , 2 , 4 - d and acetochlor between sowing and emergence 乙183 ;二183 ;扑乳油播后苗前土壤封闭防除大豆田杂草田间药效试验
Call from strawberry anthers of darslect and toyonaka were induced on ms media with 6 - ba and 2 , 4 - d , or 6 - ba and naa , and the inducing frequency was very high 当花药在附加2 0mg l6 - ba和0 5mg lnaa的培养基上进行培养时，愈伤组织诱导率最高。
When mature embryo was used as explant , as for above three lines of wheat , the optimize concentration of 2 , 4 - d for inducting calli and embryonic calli is 4 . 0 mg / l 35 ，而安农92484只能达到16 sl 。在所试的品种中对于成熟胚在2 ， 4d质量浓度为4
Chemical control using some high transporting herbicides ( mcpa , 2 , 4 - d , glyphosate , et al . ) together with some other control methods could control field horsetail effectively 采用强传导性的除草剂品种，如2甲4氯、 2 , 4 -滴、草甘膦等，并将化学防除和其他防除方法有效结合起来才可能有效地防除问荆。
However , when immature embryo was used as explant , the optimize concentration of 2 , 4 - d is different , as for yangmai 87158 and ann 98005 , it is 2 . 0 mg / l , but as for ann92484 , it is 4 . 0mg / l Omg l时出愈率和胚性愈伤组织诱导率达到最大值，而对于幼胚不同品种表现有所差异，其中杨麦87158和安农98005在2 ， 4浓度为2
The results indicated that : ( 1 ) marinated in 1 % agno3 for 15min was the optimal sterile condition of seeds . ( 2 ) 2 , 4 - d had great effects on the formation of callus , and the optimal concentration was 0 . 1mg / l 在pbi121vp7表达载体成功构建的基础上，利用质粒pbi121vp7和质粒puc19ctb上相同的ndei和xbai单限制性酶切位点，构建了pbi121ctbvp7融合表达载体。
Calli were induced on murashige - skoog ( ms ) medium containing 0 . 5mg / l to 2 . 0 mg / 1 2 , 4 - dichlorophenoxyacetic acid ( 2 , 4 - d ) and 0 . 5 rag / 1 to 1 . 0 mg / 1 6 - benzylaminopurine ( 6ba ) . a high frequency of callus induction was obtained on ms medium supplemented with 2 . 0 mg / 1 2 , 4 - d and 1 离体再生黄精根状茎中黄精凝集素的pcr扩增及序列分析表明：其与野生型凝集素比较在核苷酸序列上有2处突变，蛋白质水平上有两处氨基酸变异，分别是55位p突变为q和119位q突变p 。
