In this study , we use immunohistochemistry , electron microscope , cell culture , image analysis , mtt method and radioimmunoassay to study the localization , varies of quantitation , and possible frictions of 5 - ht and subtype of 5 - htr in human placenta . the results were as follows : 1 本研究用免疫组织化学、免疫电镜、细胞培养、图象分析、 mtt法和放射免疫等技术对5 -羟色胺及其受体亚型在胎盘绒毛中的细胞定位及对滋养层细胞增殖和激素分泌的影响进行了初步的探讨，其结果如下： 1
5 - ht induced local ca 2 + transients ( ca2 + sparks ) in cultured rat stomach fundus smooth muscle cells ( sfsmc ) was first observed by using the new fluorescent ca2 + indicator stdin and laser scanning confocal microscope . the mechanisms of initiation of ca2 + sparks and propagating ca2 + waves and their relation to e - c coupling were also discussed . 4 . the effects of reactive oxygen species on the spectral properties of the new fluorescent ca2 + indicator stdin were evaluated using four different oxidant - generating systems 应用于5一ht诱导胃底平滑肌细胞胞内游离ca2 +动员的机制研究，发现5一ht通过促进外钙内流和内钙释放使[ caz + j升高;在胃底平滑肌5一ht升高ca ， ‘ ，系通过5一htz受体介导的1p3 / ca ， ‘和dg / pkc双信使途径; 5一ht通过l型钙通道促进外钙内流。
Endocrine cells such as argyrophilic cells , 5 - hydroxytryptamine ( 5 - ht ) cells and gastrin cells were distributed in mucous and glandular epithelium of the digestive tract . the amount of endocrine cells was most in the glandular epithelium of stomach and was more in small intestine than that of large intestine and esophagus 消化道粘膜上皮和腺上皮分布着嗜银细胞、 5 - ht细胞和胃泌素细胞等内分泌细胞，其中以胃腺上皮中数量最多，小肠次之，大肠和食管零星散布。
