Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian , plant and yeast , etc . the amino acid sequence of the gene shows 99 % ( 179 / 180 ) , 98 % ( 177 / 180 ) , 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl , triticum aestivum , solatium tuberosum and bos taurus , respectively 棉花arf基因的同源性分析应用ncbi进行dna序列的相似性分析，结果表明：棉花arf基因与其它植物、动物和酵母的arf基因具有较高的同源性。棉花arf基因编码的氨基酸序列与拟南芥，小麦、马铃薯、牛的同源性分别达到99 ( 179 / 180 ) 、 98 ( 177 180 ) 。 96 ( 174 180 )杨花肠p核符吞fi曰手谷回伽皿的夭险和92 （ 168ill ） 。
Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a . a cdna clone , designated gharf ( gossypium hirsutum adp - ribosy lation factor ) , was isolated from a cotton ( gossypium hirsutum l . ) cdna library using gha27 as probe . 2 棉花arf基因的克隆选取棉花洞a雄性不育株和可育株花药cdna - aflp差别显示的差异片段gha27 ，用地高辛标记作为探针，筛选棉花洞a花药的cdna文库，克隆得到了棉花arf基因的cdna全长序列，将其命名为gharf ( gossypiumhirsutumadp - ribosylationfactor ) 。
The optimum concentration and temperature of dtpmp solution and optimum annealing temperature are discussed . in addition , hydration resistant effect of anodic oxide films treated with adp , atmp and dtpmp respectively is discussed through ft - ir , xps , afm and i - v characteristics testing 探讨了dtpmp溶液浓度、浸渍温度和热处理温度对氧化膜升压时间、比容、耐电压的影响,并找到其最佳耐水合处理工艺;同时采用ft - ir 、 xps 、 afm和i - v特性测试对adp 、 atmp 、 dtpmp三种试剂对氧化膜的耐水合处理效果进行了对比研究。
According to the analysis and identification of differential displayed proteome , two key enzymes , adp - l - glycerol - d - mannoheptose - 6 - epimerase and mannosyltransferase , were induced in different salt stress and salt shock for rt19 , which commonly participate in the biosynthesis of l - glycerol - d - manno - heptose , a major component of lipopolysaccharide 通过对rt19的盐激和盐胁迫诱导蛋白的比较分析，发现：其中12个蛋白在两种不同的胁迫条件下都可被诱导表达。进一步证实： rt19应答不同盐胁迫刺激采取了不同的反应机制，但这些机制存在共性，交织成复杂的调控网络。
Amp and adp were not significantly changed throughout the experiments , pa tended to decreased with the increases of concentrations of pollutants , nitrite - na and sodium polyphosphate could decrease atp , while methyl parathion showed a disordered atp change . aec , atp / adp and total adenylate content solely could be poor health condition markers . pa and atp are proposed as direct health condition markers of an organism to environmental stress 整个实验中未发现腺苷一磷酸( amp )和腺苷二磷酸( adp )有显著变化，磷酸精氨酸随着化合物浓度升高呈下降趋势，亚硝酸钠和多聚磷酸钠能引起腺苷三磷酸( atp )下降，而甲基对硫磷中atp变化混乱。
The results show that , the hydration resistance of anodic oxide films treated with atmp is improved greatly , the rising time decreases to 8s from 15s , which is the rising time of anodic oxide films treated with adp , and ft - ir analysis confirms that atmp treatment can inhibit oxide films from hydration effectively in boiling water 研究发现:氧化膜经atmp处理后,其耐水合特性得到很好的提高,氧化膜的升压时间从adp处理氧化膜的15s缩短到8s ; ft - ir分析证实atmp处理可有效阻滞氧化膜在沸水中的水合作用。
