If servantchoose a person for a job the unit limitation because of working property or manufacturing characteristic , cannot implement standard man - hour system , the duty when can executing adventitious is spent or the other job such as duty of integrated computation man - hour is mixed rest method , but the arrangement of relevant working hours must sign up for director branch examine and verify , after mechanism , institution is examined and approve by human affairs bureau , carry out , after by labor safeguard branch is examined and approve , company unit is carried out 若用人单位因工作性质或生产特点的限制，不能实行标准工时制度的，可以实行不定时工作制度或综合计算工时工作制等其他工作和休息办法，但相关工作时间的安排必须报主管部门审核，机关、事业单位由人事局审批后执行，企业单位由劳动保障部门审批后执行。
According to original labor department " of the duty when executing adventitious about the enterprise and duty of integrated computation man - hour examine and approve method " the 5th regulation , what duty of integrated computation man - hour uses is with week , day , season , year wait to be calculated integratedly for cycle working hours , but its are average day of working hours and average zhou gong make time answer basic and as identical as legal standard working hours 依据原劳动部《关于企业实行不定时工作制和综合计算工时工作制的审批办法》第5条的规定，综合计算工时工作制采用的是以周、日、季、年等为周期综合计算工作时间，但其平均日工作时间和平均周工作时间应与法定标准工作时间基本相同。
( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 ) ( 1 )黄瓜子叶节植株再生建立东北农业大学理学硕士学位论文一通过对比三种外植体（于叶、子叶节、下胚轴）的分化率，得出最佳外植体类型为子叶节；通过探讨pgr （ naa 、 6 ba ）浓度和配比对不定芽分化的影响，得出最佳芽诱导培养基为：卜1叭6 。
Twenty one adventitious buds from 30 root segment cultures were obtained after subculturing on medium lacking growth regulator for 90 days . the effects of hormones 6 - ba and naa on the adventitious bud differentiation were investigated and optimized . the results were showed that 6 - ba had significant effect on bud differentiation and the optimized medium for adventitious bud differentiation was ms + l . 0mg / l naa + 0 . 5mg / l 6 - ba . there were 67 adventitious buds occurred from average 30 hairy root segment cultures 90d后统计不定芽数量，平均每30个根段培养物上可获得21个不定芽；添加0 . 5mg 16 - ba能显著地促进毛状根不定芽的分化，配合使用6 - ba和naa对毛状根不定芽的分化最为有效( ms + 1 . 0mg lnaa + 0 . 5mg l6 - ba ) ， 90d后统计，平均每30个根段培养物上可获得67个不定芽。
