A late transcriptional motif , ataag , was found at - 50nt upstream of the translational start codon atg , while two tata box was located at - 112nt and - 189nt upstream of atg . a typical poly ( a ) signal was found at 12nt downstream of the translational stop codon 在hel起始密码子atg上游50位有强晚期启动子转录起始信号ataag ，在? 112位和? 189位存在两个tatabox ，但未发现早期转录信号cagt 。
Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31 , ' 30 , 28 and 25 kd in the western blotting profiles , due to the two atg initial codons at the 5 " ends of both pres2 and s gene Western杂交结果表明，感染bmpak hbmp和bmpak hbm的家蚕蛹血淋巴中均有4种不同分子量的蛋白质和鼠抗人的一抗发生免疫反应，大小约分别为31kd 、 30kd 、 zskd 、 25kd 。
The pa7 fragment was sequenced and several motifs similar to prokaryotic promoter elements such as - lobox , - 35box and up box were found . the sd sequence which was bound by ribosome and atg site were also found . the pat fragment was blast in genebank , but there is no its " homological sequence 对pa7片段的序列分析发现其距5 ’端931bp ? 1091bp处具有原核生物典型基因启动子的保守结构- 10区和- 35区，以及翻译必需的sd序列和翻译起始位点等。
Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube . there is no detectable gus activity in other floral organs , leaves and stems . these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9 通过对gus酶活性的组织化学定位分析，表明， st901基因启动子驱动gus基因特异地在成熟花粉和花粉管中表达， st901基因具有花粉表达特性；且288bp ( - 297至- 9 ) ( atg定为+ 1 )的启动子区段足以驱动gus基因在花粉中的特异表达， st901基因启动子的花粉顺式表达元件可能位于- 297至- 9之间。
2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion - S1基因：其全序列共1614bp (从起始密码子atg到s前体蛋白裂解位点) ，编码537个氨基酸，其氨基端有一编码18个氨基酸的信号肽序列，第12 13位氨基酸残基构成了信号肽的切割位点， 14 19位与111 124位氨基酸残基为s1蛋白的跨膜区域。
In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l . , pichia methanolica high - level expression systems of the genes have been constructed , and the milligram expressed protein was purified using probond resin purification system , which may result in further identification of the function of the aba binding protein . the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr , 369 bp long 3 ' - utr and poly ( a ) tail . the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr , 144 bp long 3 " - utr and poly ( a ) tail 3 - 5 - race扩增片段序列分析结果表明， abp370扩增片段的全长cdna为3449核苷酸，其中5非翻译区为876个核苷酸， 3非翻译区为369个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子tga ，含有一个编码768个氨基酸残基的开放阅读框架( 2304bp ) ； abp640扩增片段的全长cdna为1012核苷酸，其中5非翻译区为88个核苷酸， 3非翻译区为144个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子taa ，含有一个编码260个氨基酸残基的开放阅读框架( 780bp ) 。
The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites , four conserved cysteine residues , two out - of - frame atg initiation codons in the 5 " untranslated region , and a signal peptide consisting of 66 amino acids . when it was compared with the published sequence of chil - 15 cdna , 7 mutant sites were found , and 5 amino acids were changed in predicted amino acids , which indicated that chil - 15 may be polymorphic 结果显示，本研究所用白来航鸡il - 15cdna5 ’非编码区有两个框外atg起始密码子，开放阅读框由564bp组成，编码187个氨基酸，其中n末端信号肽含有66个氨基酸残基，在第48 、 149和166位的天冬酰胺残基上有三个潜在的n -糖基化位点。
This implied that the 31 kd initiated from atg codon of pres2 gene , the 30 kd encoded by the s gene was glycosylated form of s protein , the 25 kd initiated from atg codon of the pres2 gene was disassembled form of 31 kd and the 28 kd was a glycosylated form of25kd . under optimal condition the expression level of foreign gene was considerably affected on hosts ( silkworm race ) , the highest difference of expression level in various races can reach up to 8 times 推测31kd处的条带为起始于presz起始密码子atg的表达产物，同时具有pres ： z和s抗原性， 30kd处的杂交条带为起始于s基因起始密码子atg表达产物的糖基化形式， 25kd的条带可能是中蛋白的降解产物，而28kd的条带可能25kd条带的糖基化形式。
The orf1 protein of senv - h12 - 1 isolate was consisted of 675 amino acids and 92 amino - terminal amino acids less than senv - h ( ax025838 ) , it had insertion in two sites also . the orf1 protein of senv - h 12 - 2 isolate was 7 amino acids less than senv - h ( ax025838 ) because of deletion . because the first start code was mutated , the orf2 protein of senv - h 12 - 2 isolate started from the second atg code , so that it was 7 amino acids less than senv - h ( ax025838 ) from its n - terminus 两个senv - h亚型分离株的情况与已发表的博士学位论文: sen病毒分子病毒学和流行病学研究对sen琴h ( ax025838 )株分析的结果有所不同: senv - h12一1分离株的orfi仅由675个氨基酸组成，比senv - h ( axo25838 )的orfi少n一末端的92个氨基酸，此外在2个位点上还存在插入: senv - h12一2分离株在一些位点上的缺失造成o盯1蛋白比senv - h ( axo25838 )少7个氨基酸，由于第一个起始密码子发生突变，采用位于下游的第二个起始密码子使得o即2蛋白比senv - - h ( axo25838 )少n一末端的7个氨基酸。
