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Avec des cham qui repr sentent peine 7 de la surface cultiv e du monde , notre agriculture nourrit une population qui d pa e un cinqui me de la population mondiale 我们的农业以不到世界百分之七的耕地面积,却养活了超过世界五分之一的人口。
Avec des champs qui repr sentent peine 7 de la surface cultiv e du monde , notre agriculture nourrit une population qui d passe un cinqui me de la population mondiale 我们的农业以不到世界百分之七的耕地面积,却养活了超过世界五分之一的人口。
Based on a 3 . 1kb pst i fragment of genomic dna of a wild s . avermitilis , a 1 . 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3 . 1 kb fragment , then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401 . competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively 以含有avec基因的3 . 1kb基因组dnapsti片段为基础,将1 . 5kb的安普霉素抗性基因片段插入到avec基因中的sphi酶切位点,再将此插入失活的avec基因片段连接到具有接合转移功能(含有orit基因)的链霉菌-大肠杆菌穿梭质粒phjl401的多克隆位点区,由此得到重组质粒pc05 。
In this study , the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way , four " 1 " components were lost and only " 2 " components , the potential precursor of ivermectin , were accumulated 将该基因簇中的aved基因通过插入外源的安普霉素抗性基因片段使其失活,导致发酵产物中4个a组分(不需要的组分)的消失;将基因簇中的avec基因通过同样手段,使其失活,导致发酵产物中4个“ 1 ”组分的消失,而主要积累“ 2 ”组分(进一步改造可成为伊维菌素的前体b _ 2组分) 。
Cries le fcondateur , tripping in , my friend monsieur moore , that most accomplished traveller i have just cracked a half bottle avec lui in a circle of the best wits of the town , is my authority that in cape horn , ventre biche , they have a rain that will wet through any , even the stoutest cloak 授精业者171大声插嘴曰, “吾友穆尔172先生乃一非凡之旅人适才吾与彼173曾共饮酒半瓶,座中有市内博学之士,彼据可靠消息告知,霍恩岬角,雨势猛烈174 ,致使所有外衣无论何等结实,均已湿透。
Born in thailand , obtained his b . arch from chulalongkorn university , bangkok in 1982 , m . arch with distinction from university of michigan ann arbor , u . s . a . in 1986 and doctorat de linstitut national des langues et civilisations orientales , tres honorable avec felicitations , paris in 2004 . his ph . d . research focuses on buddhism in architecture 生于泰国,一九八二年在曼谷chulalongkorn大学获得建筑学士,一九八六年于美国密西根大学以前几名的成绩毕业取得硕士,最后则在两千零四年于巴黎以极度优异的成绩获得国家东方语言与文化学院之博士,博论著重于建筑中的佛教精神。