In immediate death group , the gray value of bfgf positive nerve cells is the smallest , which had magnificant difference with other experimental groups ( p < 0 Bfgf免疫组织化学染色。即刻死亡组hfgf阳性神经细胞灰度值最小，与其它各实验组相比有显著差异（ p o
Induced differentiation is the hot spot of nscs research all the time . among factors affecting the differentiation of nscs , egf and bfgf are the most thorough and extensive researched Ad的毒性也可为许多因子如雌激素等所拮抗，因此它们在ad的治疗中发挥着重要作用。
2 . the proapoptotic effects result from the reduction of erk , activation of jnk during treatment of bfgf and p38 also has some role in this process Bfgf作用于u2 os细胞引起的erk活性抑制和jnk长时程激活共同介导了u2 os细胞凋亡的发生， p38也在一定程度上参与了这个过程。
Bfgf is essential for the proliferation and differentiation of neural stem cells , it can also affect the process of expression of rara mrna induced by atra or bmp - 2 ; 2 Bfgf在神经干细胞增殖与分化中都起着必不可少的营养作用， bfgf能影响al ， ra 、 bmp一2对raramrna表达的诱导
In the later experiments dmem - lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem - lg / atra ( 150mg / ml ) was used as the neuronal induction media 在以后的实验中， bfgf瞩0n才rnl ）被用来作为前诱导剂，人ttiho50略inl ）被用来作为诱导剂。
There appears obvious apoptotic peak on dna content curve of facs analysis . and as the time of bfgf treatment increases , percent of apoptotic cells increases . 3 在流式细胞术dna含量曲线上显示出明显的亚二倍体峰（凋亡细胞峰入并且随着bfgf作用时间的延长， u2 os细胞凋亡百分比逐渐增加。
In this study , the putative eg cell culture media was supplemented with lif , bfgf , scf and igf - 1 . as a result , the ideal dose of lif , bfgf , scf and igf - 1 was : 100iu / ml , 10ng / ml , 10ng / ml , 10ng / ml 而随着培养时间的延长，添加lif 、 bfgf 、 scf 、及igf - 1组表现的优势越来越明显，对促进类eg集落的持续增多效果最佳。
Bfgf can promote death of u 2 - os osteosarcoma cell line , at the same time , the type of cell death is apoptosis . and the proapoptot - ic effect of bfgf is concentration and time dependent Bfgf盲够诱导u2 os细胞的死亡，同时u2 os细胞的死亡是以凋亡的形式发生，并且bfgf诱导u2 os细胞凋亡的作用是时间和浓度依赖性的。
Although the growth factors did have a positive effect on keeping the undifferent state of eg colonies cultured on sto feeders , we can not get eg colonies when pgcs were cultured on 0 . 1 % gelatin in medium supplemented with lif , scf and bfgf 而培养板底只铺有0 . 1的明胶时，即使培养液中加入生长因子也没有形成典型的eg集落。
Therefore , this project intend to explore mechanism of apoptosis mediated by protein tyrosine kinase ( ptk ) pathway by study of the role of erk1 / 2 , jnk and p38 in apoptosis of u 2 - os cell line induced by bfgf 本课题欲通过研究erk1 2 、 jnk 、 p38在bfgf诱导u2 - os细胞凋亡中的作用来探讨酪氨酸蛋白激酶途径参与细胞凋亡调控的作用机制。
